• Quick Order

Mycoplasma Growth Supplement, 10x solution


Mycoplasma Growth Supplement is used in broth and agar media to isolate and grow various mycoplasma species. At ATCC the 10x solution is an ingredient in ATCC Medium 988 (Spiroplasma SP-4 Medium).


Product category

L-Alanine, 0.025 g/L

L-Arginine, 0.05787 g/L

L-Aspartic Acid, 0.03 g/L

L-Cysteine•HCL•H2O, 0.26 g/L

L-Cystine, 0.02 g/L

L-Glutamic Acid, 0.075 g/L

L-Glutamine, 0.1 g/L

Glycine, 0.05 g/L

L-Histidine HCl•H2O, 0.02 g/L

Trans-4-Hydroxy-L-Proline, 0.01 g/L

L-Isoleucine,  0.02 g/L

L-Leucine, 0.06 g/L

L-Lysine•HCl, 0.07 g/L

L-Methionine, 0.015 g/L

L-Phenylalanine, 0.025 g/L

L-Proline, 0.04 g/L

L-Serine, 0.025 g/L

L-Threonine, 0.03 g/L

L-Tryptophan, 0.01 g/L

L-Tyrosine, 0.04 g/L

L-Valine, 0.025 g/L

L-Ascorbic Acid, 0.05 g/L

PABA, 0.00005 g/L

D-Biotin, 0.00001 g/L

Choline Chloride, 0.0005 g/L

Coenzyme A•Na, 0.0025 g/L

Cocarboxylase, 0.001 g/L

2’-Deoxyadenosine, 0.01 g/L

2’-Deoxyguanosine, 0.01 g/L

2’-Deoxycytidine•HCl, 0.0116 g/L

Flavin Aadenine Dinucleotide•2Na, 0.000106 g/L

Folic Acid, 0.00001 g/L

myo-Inositol, 0.00005 g/L

5-Methyldeoxycytidine, 0.0001 g/L

β-NAD, 0.007 g/L

β-NADP•Na,0.001 g/L

Niacinamide, 0.000025 g/L

Nicotinic Acid, 0.000025 g/L

D-Pantothenic Acid (hemicalcium), 0.00001 g/L

Pyridoxal•HCl, 0.000025 g/L

Pyridoxine•HCl, 0.000025 g/L

Riboflavin, 0.00001 g/L

Thiamine•HCl, 0.00001 g/L

Thymidine, 0.01 g/L

Uridine-5-Triphosphate•Na, 0.001 g/L

Calcium Chloride (Anhydrous), 0.2 g/L

Magnesium Sulfate (Anhydrous), 0.09769 g/L

Potassium Chloride, 0.4 g/L

Sodium Acetate (Anhydrous), 0.05 g/L

Sodium Chloride, 6.8 g/L

Sodium Phosphate Monobasic (Anhydrous), 0.122 g/L

D-Glucose, 1.0 g/L

Phenol Red•Na, 0.02124 g/L

Glutathione, 0.01 g/L

D-Glucuronic Acid•Na, 0.00388 g/L

Cholesterol, 0.0002 g/L

Tween 80, 0.005 g/L

Well-defined supplement for mycoplasma culture media. Add aseptically to prepared medium.
100 mL
Storage conditions
2°C to 8°C
Buy Now
Price: $105.00 EA
Discounts may be available for our fellow nonprofit organizations.

Generally ships within 1-3 business days


ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
Mycoplasma Growth Supplement is used in broth and agar media to isolate and grow various mycoplasma species. At ATCC the 10x solution is an ingredient in ATCC Medium 988 (Spiroplasma SP-4 Medium).

Handling information

Handling procedure
  1. Add Mycoplasma Growth Supplement to Spiroplasma Medium SP-4 according to the following formulation:

    Basal Medium:
    PPLO Broth w/o Crystal Violet (BD 255420) 11.0 g
    Tryptone (BD 211705) 10.0 g
    Noble Agar (for solid medium) 8.0 g
    DI Water 525.0 mL

  2. Adjust pH to 7.4 to 7.5. Gently boil to dissolve agar, if necessary. Autoclave for 15 minutes at 121°C. Cool agar medium to 50 to 55°C, broth to 55°C or cooler. Aseptically add additional ingredients (below).

    Additional Ingredients (all sterile):
    Mycoplasma Growth Supplement 10x 50.0 mL
    Dextrose Solution 5% 100.0 mL
    Yeast Extract Solution 25% 35.0 mL
    TC Yeastolate Solution 2% (BD 255772) 100.0 mL
    Phenol Red Solution 0.1% 20.0 mL
    Fetal Bovine Serum (heat-inactivated) 170.0 mL

  3. Aseptically add these ingredients to the basal medium, mixing well by swirling. Adjust final pH to 7.25 to 7.4. Dispense as required.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at


Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.



US and Puerto Rico


Outside the US


Hours of Operation

9:30am - 5:30pm
US Eastern Time