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Desulfotomaculum nigrificans (Werkman and Weaver) Campbell and Postgate


Product category
Product type
Strain designation
[IFO 13698, NCIB 8395; Delft 74T]
Type strain
Isolation source
Geographical isolation
Netherlands; Delft
Product format
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Sulfate reducer

Handling information

Handling procedure
1. Open vial according to enclosed instructions.

2. Under anaerobic conditions, withdraw 0.5 ml of the #1249 broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.

3. Aseptically transfer this aliquot back into the broth tube and inoculate a #260 (blood agar) plate to be incubated anaerobically.  An aerobic blood plate may also be streaked to check for purity.

4. Incubate tubes and plate under an anaerobic atmosphere at 55oC.  Incubate blood plate aerobically at 37oC.

5. Within 24 to 48 hours, growth is evident by moderate to good turbidity in the broth with sediment in the bottom of the tube.  No growth occurs on the blood agar plate incubated aerobically.  Once growth is achieved, transfer the culture to fresh tubes of #1249 broth.  This culture does not grow well on agar.


          Anaerobic conditions for transfer may be obtained by the following:

·Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

          Anaerobic conditions for incubation may be obtained by any of the following:

·Loose screw caps on test tubes in an activated anaerobic gas pack jar, or

·Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

Handling notes
Always use freshly prepared anaerobic media.

          The best results have been obtained using the gassing cannula system.  For reviving the cultures initially, an anaerobic jar is not recommended.  Once the culture has been established, an anaerobic jar can be used if the inoculum is 20% or greater.

          Either 100% N2 or 80% N2-10% CO2-10% H2 can be used as the anaerobic gas for culturing this organism.

          Once growth has been obtained, this culture is fairly easy to maintain if transferred every other day.  A culture that has good growth and is fresh can be maintained at 4oC for up to a week.  The cells can be stored at –70 to –80oC by growing a large volume in ATCC Medium #1249, harvesting the cells and then mixing the cell pellet in an equal volume of fresh #1249 and 20% glycerol (10% final concentration).  Dispense the cells into vials (approximately 0.5 to 1.0 ml per vial) and freeze rapidly.  Both the #1249 broth and glycerol need to be pre-reduced.  This may be accomplished by adding 0.1 ml (for each 5 to 6 ml medium) of a 1.5% sodium sulfide solution.   

          Additional information on this culture is available on the ATCC® web site at


Deposited as
Desulfotomaculum nigrificans (Werkman and Weaver) Campbell and Postgate
LL Campbell
Chain of custody
ATCC <-- LL Campbell <-- NCIB 8395 <-- R.L. Starkey Delft 74T
Type of isolate

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Campbell LL, Postgate JR. Classification of the spore-forming sulfate-reducing bacteria. Bacteriol. Rev. 29: 359-363, 1965. PubMed: 5826606

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

type strain

fresh water, Delft

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