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Mycoplasma pulmonis

19612

Mycoplasma pulmonis strain [Ash, PG34; L3] is a bacterial type strain that was isolated from the lung lesion of a rat. Growth of this strain is best achieved if broth cultures are allowed to incubate aerobically and the plates in 5% CO2.
Product category
Bacteria
Strain designation
[Ash, PG34; L3]
Type strain
Yes
Isolation source
Lung lesion of rat
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Handling information

Medium
Temperature
37°C
Atmosphere
Broth: Aerobic
Plates: 5% CO2
Handling procedure
  1. Follow instructions as suggested for the culturing of Mollicutes:

                PROCEDURES FOR PROPAGATING MOLLICUTES:

    1. Open the thawed vial according to the enclosed instructions.
    2. Make serial dilutions by transferring 0.5 mL from the original tube to a tube to a tube containing 4.5 mL of #243 broth. Repeat process by transferring 0.5 mL from the second to third tube, etc.
    3. Plates may be inoculated to check colony morphology.  You can also spot each dilution on the surface of plate to determine the number of colony forming units. However, not all strains do well on solid medium. 
    4. Incubate all broth tubes at 37°C, aerobic and plates at 37°C, 5% CO2. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation. 
    5. Growth in broth will be indicated by increased turbidity. Note: The turbidity may be difficult to see. Placing tubes in front of a light source will help to see the slight cloudiness. A live/dead stain will confirm cells present.
Handling notes

Many strains will die out rapidly once acid or alkaline conditions are reached. 

Growth is best achieved if the broths are allowed to incubate aerobically and the plates in 5% CO2.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Mycoplasma pulmonis (Sabin) Freundt
Depositors
RG Wittler
Chain of custody
ATCC <-- RG Wittler <-- D.G. ff. Edward PG 34 <-- E. Freundt <-- E. Klieneberger L3
Type of isolate
Animal
Cross references
GenBank M23941 M.pulmonis 16S ribosomal RNA small subunit.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Sneath PH, Skerman VB. A list of type and reference strains of bacteria. Int. J. Syst. Bacteriol. 16: 1-134, 1966.

Edward DG. A suggested classification and nomenclature for organisms of the pleuropneumonia group. Int. Bull. Bacteriol. Nomencl. Taxon. 5: 85-93, 1955.

Edward DG, Freundt EA. Type strains of species of the order Mycoplasmatales, including designation of neotypes for Mycoplasma mycoides subsp. mycoides, Mycoplasma agalactiae subsp. agalactiae, and Mycoplasma arthritidis. Int. J. Syst. Bacteriol. 23: 55-61, 1973.

Edward DG, Freundt EA. The classification and nomenclature of organisms of the pleuropneumonia groups. J. Gen. Microbiol. 14: 197-207, 1956. PubMed: 13306904

Klieneberger E. Pleuropneumonia-like organisms of diverse provenance: some results of an enquiry into methods of differentiation. J. Hyg. 38: 458-476, 1938.

View All Curated Citations for this Product

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