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Peredibacter starrii (Seidler et al.) Davidov and Jurkevitch


Product category
Strain designation
A3.12 [ICPB 3279]
Type strain
Isolation source
Geographical isolation
United States; California
Product format
Storage conditions
2°C to 8°C
Mission Collection Item
This is a Mission Collection Item


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

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Detailed product information




Predator of Pseudomonas putida ATCC 12633

Handling information

Grown with Pseudomonas putida ATCC® 12633™
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a tube with approximately 2.0 mL of ATCC Medium #137, withdraw 0.5 mL and rehydrate the entire pellet. 
  3. Transfer the suspension back into the tube and mix well.
  4. Incubate the tube on a reciprocal shaker at 30°C for 6 hours.
  5. After culture has incubated, melt tubes containing 2.5 mL each of 0.6% semi-solid agar, and place in a 45°C water bath.
  6. Add 0.2 to 0.3 mL of the host/predator suspension to each melted tube and pour over the surface of pre-warmed #137 plates. 
  7. Incubate plates at 30°C for three to six days.  After this time, plaques should be visible.  Observe daily until plaques or clearing are seen.  When a small loopful is observed microscopically under wet mount, you should observe free-swimming Bacteriovorax cells, host cells, and host cells with attached Bacteriovorax.
  8. Scrape off overlay into a sterile testtube and centrifuge at low speed to sediment the agar and cellular debris.  The Bacteriovorax cells will be in the supernatent.  Check for very tiny, rapidly moving cells under a wet mount.
Handling notes
Inoculating a Nutrient Agar slant with some of the mixed culture for subsequent overnight incubation will allow the Pseudomonas sp. to propagate for use in further inoculations.  It should be used as a 24 hour culture.
A pure culture of the host strain ATCC® 12633™ Pseudomonas putida is also available and may also be used instead of trying to propagate the host from the mixed culture.
Additional information on this culture is available on the ATCC® web site at


Deposited as
Bdellovibrio bacteriovorus Stolp and Starr
H Stolp
Type of isolate
Cross references
GenBank M61231 Bdellovibrio starrii 16S rRNA gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Seideler RJ, et al. Molecular heterogeneity of the Bdellovibrios: evidence of two new species. J. Bacteriol. 109: 209-217, 1972. PubMed: 4621626

Stolp HJ, Starr MP. Bdellovibrio bacteriovorus gen. et sp. n., a predatory, ectoparasitic and bacteriolytic microorganism. Antonie van Leeuwenhoek 29: 217-248, 1963.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Baer ML, et al. A proposal for the reclassification of Bdellovibrio stolpii and Bdellovibrio starrii into a new genus, Bacteriovorax gen. nov., as Bacteriovorax stolpii comb. nov. and Bacteriovorax starrii comb. nov., respectively. Int. J. Syst. Evol. Microbiol. 50: 219-224, 2000. PubMed: 10826807

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