Paenibacillus lentimorbus (Dutky) Pettersson et al.
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2. Using a single tube of #20 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use 0.3 to 0.5 ml of the #20 suspension to overlay a #260 agar slant and streak a #260 plate.
5. Incubate the slant and plate at 26oC for 3 to 5 days.
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Haynes WC, et al. Preservation of infectious milky disease bacteria by lyophilization. J. Insect Pathol. 3: 55-61, 1961.
Roberts MS, et al. Bacillus vallismortis sp. nov., a close relative of Bacillus subtilis, isolated from soil in Death Valley, California. Int. J. Syst. Bacteriol. 46: 470-475, 1996. PubMed: 8934905
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Pettersson B, et al. Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.. Int. J. Syst. Bacteriol. 49: 531-540, 1999. PubMed: 10319474
Rippere KE, et al. Bacillus popilliae and Bacillus lentimorbus, bacteria causing milky disease in Japanese beetles and related scarab larvae. Int. J. Syst. Bacteriol. 48: 395-402, 1998.