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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
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Georg LK, et al. Identification of species of Actinomyces. J. Bacteriol. 88: 477-490, 1964. PubMed: 14203367
Pine L, Hardin H. Actinomyces israelii, a cause of lacrimal canaliculitis in man. J. Bacteriol. 78: 164-170, 1959. PubMed: 14433042
Buchanan BB, Pine L. Characterization of a propionic acid producing actinomycete, Actinomyces propionicus, sp. nov.. J. Gen. Microbiol. 28: 305-323, 1962. PubMed: 13874228
Pine L, Georg LK. Reclassification of Actinomyces propionicus. Int. J. Syst. Bacteriol. 19: 267-272, 1969.
Charfreitag O, et al. Reclassification of Arachnia propionica as Propionibacterium propionicus comb. nov.. Int. J. Syst. Bacteriol. 38: 354-357, 1988.