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Corynebacterium glutamicum (Kinoshita et al.) Abe et al.


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Corynebacterium glutamicum strain 534 [NCIB 10025] is a whole-genome sequenced bacterial type strain. The culture was isolated from sewage and is known to produce glutamic acid. It can be used as a transformation host.
Product category
Strain designation
534 [NCIB 10025]
Type strain
Genome sequenced strain
Isolation source
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces glutamic acid L-glutamate, L-glutamic acid, glutamate
Transformation host


Genome sequenced strain
Lysine uptake and exchange
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate the tubes and plate at 37°C for 24 hours.
Handling notes
ATCC Medium 18: Trypticase Soy Agar/Broth can also be used for growth and maintenance of this strain.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Micrococcus glutamicus Kinoshita et al.
Kyowa Ferm. Ind. Co., Ltd.
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
Type of isolate
Cross references
GenBank Y09578 C.glutamicum leuB gene.
GenBank M16175 C.glutamicum 5S rRNA.
GenBank X93513 C.glutamicum amt gene.
GenBank X93514 C.glutamicum betP gene.
GenBank X95649 C.glutamicum ORF4 gene.
GenBank Y09163 C.glutamicum putP gene.
GenBank X81379 C.glutamicum dapE gene and orf2.
GenBank X85965 C.glutamicum ORF3 and aroP gene.
GenBank A78796 Sequence 1 from Patent EP0563527.
GenBank A78797 Sequence 2 from Patent EP0563527.
GenBank A78798 Sequence 3 from Patent EP0563527.
GenBank X89850 C.glutamicum DNA for attB region.
GenBank X78491 C.glutamicum (ATCC 13032) aceB gene.
GenBank X89084 C.glutamicum pta gene and ackA gene.
GenBank Y09548 Corynebacterium glutamicum pyc gene.
GenBank X96580 C.glutamicum panB, panC & xylB genes.
GenBank Y13221 Corynebacterium glutamicum glnA gene.
GenBank AJ238250 Corynebacterium glutamicum ndh gene.
GenBank Y17592 Corynebacterium glutamicum accDA gene.
GenBank AJ012293 Corynebacterium glutamicum ilvD gene.
GenBank X90356 C.glutamicum DNA for promoter fragment F1.
GenBank X90357 C.glutamicum DNA for promoter fragment F2.
GenBank X90358 C.glutamicum DNA for promoter fragment F10.
GenBank X90359 C.glutamicum DNA for promoter fragment F13.
GenBank X90360 C.glutamicum DNA for promoter fragment F22.
GenBank X90361 C.glutamicum DNA for promoter fragment F34.
GenBank X90362 C.glutamicum DNA for promoter fragment F37.
GenBank X90363 C.glutamicum DNA for promoter fragment F45.
GenBank X90364 C.glutamicum DNA for promoter fragment F64.
GenBank X90365 C.glutamicum DNA for promoter fragment F75.
GenBank X60312 C.glutamicum lysI gene for L-lysine permease.
GenBank X81191 C.glutamicum gluA, gluB, gluC and gluD genes.
GenBank X75504 C.glutamicum aceA gene and thiX genes (partial).
GenBank Y16642 Corynebacterium glutamicum lpd gene, complete CDS.
GenBank X70959 C.glutamicum gene leuA for isopropylmalate synthase.
GenBank X86157 C.glutamicum argC, argJ, argB, argD, and argF genes.
GenBank AJ004934 Corynebacterium glutamicum dapD gene, complete CDS.
GenBank X67737 C.glutamicum dapB gene for dihydrodipicolinate reductase.
GenBank X71489 C.glutamicum icd gene for monomeric isocitrate dehydrogenase.
GenBank M25819 C.glutamicum phosphoenolpyruvate carboxylase gene, complete cds.
GenBank X59404 Corynebacterium glutamicum, gdh gen for glutamate dehydrogenase.
GenBank U31281 Corynebacterium glutamicum biotin synthase (bioB) gene, complete cds.
GenBank E04376 DNA sequence encoding corynebacterium glutamicum isocitric acid lyase.
GenBank AF189147 Corynebacterium glutamicum ISCg2 transposase (tnp) gene, complete cds.
GenBank AB055218 Corynebacterium glutamicum gene for superoxide dismutase, complete cds.
GenBank AJ306418 Corynebacterium glutamicum ctaE gene, qcrC gene, qcrA gene and qcrB gene.
GenBank L01508 Corynebacterium glutamicum threonine dehydratase (ilvA) gene, complete cds.
GenBank AF005635 Corynebacterium glutamicum glutamine synthetase (glnA) gene, complete cds.
GenBank AJ269506 Corynebacterium glutamicum pck gene for phosphoenolpyruvate carboxykinase.
GenBank U43535 Corynebacterium glutamicum multidrug resistance protein (cmr) gene, complete cds.
GenBank U43536 Corynebacterium glutamicum heat shock, ATP-binding protein (clpB) gene, complete cds.
GenBank AJ006985 Streptomyces glaucescens GLA.0 gene cluster for 5'-hydroxystreptomycin biosynthesis.
GenBank E04377 DNA sequence encoding corynebacterium glutamicum isocitric acid lyase N-terminal fragment.
GenBank AF112536 Corynebacterium glutamicum ribonucleotide reductase beta-chain (nrdF) gene, complete cds.
GenBank AJ300822 Corynebacterium glutamicum secE gene, nusG gene, rplK gene, rplA gene and partial pkwR gene.
GenBank AF116184 Corynebacterium glutamicum L-aspartate-alpha-decarboxylase precursor (panD) gene, complete cds.
GenBank U35023 Corynebacterium glutamicum thiosulfate sulfurtransferase (thtR) gene, partial cds, acyl CoA carboxylase (accBC) gene, complete cds.
GenBank AJ306417 Corynebacterium glutamicum partial nrdF gene for ribonucleotide reductase beta-subunit and ctaD gene for cytochrome aa3 oxidase subunit I.
GenBank L09232 Corynebacterium glutamicum acetohydroxy acid synthase (ilvB) and (ilvN) genes, and acetohydroxy acid isomeroreductase (ilvC) gene, complete cds.
GenBank AF112535 Corynebacterium glutamicum putative glutaredoxin NrdH (nrdH), NrdI (nrdI), and ribonucleotide reductase alpha-chain (nrdE) genes, complete cds.
GenBank M89931 Corynebacterium glutamicum beta C-S lyase (aecD) and branched-chain amino acid uptake carrier (brnQ) genes, complete cds, and hypothetical protein Yhbw (yhbw) gene, partial cds.
GenBank U13922 Corynebacterium glutamicum putative type II 5-cytosoine methyltransferase (cglIM) and putative type II restriction endonuclease (cglIR) and putative type I or type III restriction endonuclease (clgIIR) genes, complete cds.
GenBank BA000036 Corynebacterium glutamicum ATCC 13032 DNA, complete genome.

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Broer S, Kramer R. Lysine uptake and exchange in Corynebacterium glutamicum. J. Bacteriol. 172: 7241-7248, 1990. PubMed: 2123868

Sadao A, et al. Method of producing l-glutamic acid. US Patent 3,002,889 dated Oct 3 1961

Sadao A, et al. Microbiological production of amino acid by reductive amination. US Patent 3,220,929 dated Nov 30 1965

Tsuchida T, et al. Process for producing L-amino acids by fermentation. US Patent 5,705,370 dated Jan 6 1998

Kinoshita S, et al. Method of producing L-glutamic acid by fermentation. US Patent 3,003,925 dated Oct 10 1961

View All Curated Citations for this Product

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