MicroQuant™ Penicillium rubens, low CFU (Pack of 5)

MicroQuant™ strains are best-in-class controls developed to support USP General Chapters. These precisely quantitated controls are provided in a single-use format that rehydrates immediately and is easy to use and convenient to store. MicroQuant™ strains are direct from ATCC—passage zero.

MicroQuant™ Penicillium rubens, low CFU (11709-LQ-PACK™) is provided as spores.

High-yielding, sporulating, penicillin strain

The strain was re-identified as Penicillium rubens, a close sister of Penicillium chrysogenum, based on the multigene sequence analysis.

1. Remove the desired number of cryopreserved reference culture vials (gray crimp) and rehydration buffer vials (green crimp) from 2-8°C storage and place into a biosafety cabinet. One vial of rehydration buffer should be used for each vial of reference culture.
2. Uncap each vial. Add 1.0 mL of rehydration buffer to the reference culture vial. Recap the vial and allow the pellet to dissolve for 30 seconds. 
3. Vortex on the high setting for 30 seconds to ensure the suspension is well mixed. The suspension is now useable and contains 100 to 1000 CFU per 1.0 mL (10 to 100 CFU per 100 µL). The suspension can be used immediately or stored at 2-8°C for up to 8 hours. Note: Before plating, gently pipette the suspension up and down five times to ensure thorough mixing. If the suspension has been left standing for more than five minutes, or if it was stored at 2–8°C after rehydration, vortex the suspension again to ensure uniformity prior to use.
4. Uncap the vial and pipette 100 µL of the resuspended reference culture directly on a non-selective media agar plate. If following a specific pharmacopeial chapter, use the appropriate media specified. Note: ATCC quantitation results were obtained using 100 mm diameter Tryptic Soy Agar plates with 1.5% Tryptone, 0.5% Soytone, 0.5% Sodium Chloride, and 1.5% Agar (plates were procured from Teknova). 
5. Use a sterile plate spreader (not provided) to spread the sample evenly over the surface of the media plate. Incubate the plates in an incubator at the appropriate temperature (see the product page or product sheet for details). If following a specific pharmacopeial chapter, use the incubation conditions specified. 
6. Following incubation, count the number of visible colonies on the plate using a standard colony counting procedure.

For applications outside of compendial assays, please refer to the product page for ATCC 11709 for standard growth conditions.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.