Photobacterium phosphoreum (Cohn) Beijerinck
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2. Open vial according to enclosed instructions.
3. Using a single tube of #101 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
4. Aseptically transfer this aliquot back into the broth tube. Mix well.
5. Incubate the broth tube immediately for four to five days at 18oC.
6. Transfer can now be made to other tubes of broth, slants, and/or plates.
This strain will grow on Marine Broth 2216 (BD 279110) and Marine Agar 2216 (BD 212185), but Photobacterium Medium is recommended for demonstrating luminescence. To check for luminescence, inoculate a slant, leaving cap loose. Incubate at optimal temperature until growth is observed. Then, hold in the dark for 10 minutes. If no luminescence is detected, reincubate and check again after 24-48 additional hours. When maximum luminescence is obtained, stopper tightly.
Colonies appear smooth, slightly irregular, colorless, pinpoint and translucent.
This strain needs to establish good growth in broth culture before additional transfers are made. Additional incubation
may be required for growth on solid media.
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Hendrie MS, et al. The identification, taxonomy and classification of luminous bacteria. J. Gen. Microbiol. 64: 151-169, 1970.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.