Photobacterium phosphoreum (Cohn) Beijerinck


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Strain designation
[NCMB 1282]
Type strain
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information




This strain is temperature sensitive
Rehydrate with pre-chilled medium and incubate immediately at 15-20C.
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Note: Rehydrate with pre-chilled medium and incubate immediately at 15-20C.
Handling procedure
1. Pre-chill all media by placing at 4oC for 30 minutes.

2. Open vial according to enclosed instructions.

3. Using a single tube of #101 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.

4. Aseptically transfer this aliquot back into the broth tube.  Mix well.

5. Incubate the broth tube immediately for four to five days at 18oC.

6. Transfer can now be made to other tubes of broth, slants, and/or plates.

Handling notes
This strain is temperature sensitive.  You MUST use chilled media for rehydration or the cells may be damaged.  Place tubes at the proper temperature immediately.  Temperatures of 20oC or above should be avoided.  Store or transfer immediately.  Do not let them stand at room temperature.

        This strain will grow on Marine Broth 2216 (BD 279110) and Marine Agar 2216 (BD 212185), but Photobacterium Medium is recommended for demonstrating luminescence.  To check for luminescence, inoculate a slant, leaving cap loose.  Incubate at optimal temperature until growth is observed.  Then, hold in the dark for 10 minutes.  If no luminescence is detected, reincubate and check again after 24-48 additional hours.  When maximum luminescence is obtained, stopper tightly.

        Colonies appear smooth, slightly irregular, colorless, pinpoint and translucent.

        This strain needs to establish good growth in broth culture before additional transfers are made.  Additional incubation

may be required for growth on solid media.


Deposited as
Bacterium phosphoreum (Cohn) Molisch
FH Johnson
Chain of custody
ATCC <-- FH Johnson <-- A. Kluyver
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Cross references
GenBank M29761 P.phosphoreum Arg-tRNA and His-tRNA genes.
GenBank X12975 Photobacterium phosphoreum DNA for tRNA-Pro and tRNA-His.
GenBank X12976 Photobacterium phosphoreum DNA for tRNA-Pro and tRNA-His.
GenBank AF136386 Photobacterium phosphoreum gyrase B (gyrB) gene, partial cds.
GenBank X74687 Photobacterium phosphoreum (ATCC 11040T) gene for 16S ribosomal RNA.

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Hendrie MS, et al. The identification, taxonomy and classification of luminous bacteria. J. Gen. Microbiol. 64: 151-169, 1970.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

type strain

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