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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Anaerobic conditions for transfer may be obtained by either of the following:
Anaerobic conditions for incubation may be obtained by any of the following:
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De Ley J, Swings J. Phenotypic description, numerical analysis, and proposal for an improved taxonomy and nomenclature of the genus Zymomonas Kluyver and van Niel 1936. Int. J. Syst. Bacteriol. 26: 146-157, 1976.
Bajpai PK, et al. Effects of calcium chloride concentration on ethanol production and growth of immobilized Zymomonas mobilis. J. Ferment. Technol. 63: 199-203, 1985.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Rogers PL, et al. Kinetics of alcohol production by Zymomonas mobilis at high sugar concentrations. Biotechnol. Lett. 4: 165-179, 1979.
Lee KJ, et al. Ethanol production by Zymomonas mobilis in continuous culture at high glucose concentrations. Biotechnol. Lett. 4: 421-426, 1979.