Burkholderia gladioli Yabuuchi et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30°C for 24 hours.
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Dye DW, et al. International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and pathotype strains. Rev. Plant Pathol. 59: 153-168, 1980.
Ballard RW, et al. Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. J Gen Microbiol 60: 199-214, 1970. PubMed: 5488054
Validation list no. 45. Int. J. Syst. Bacteriol. 43: 398-399, 1993.
Yabuuchi E, et al. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. [published erratum appears in Microbiol Immunol 37: 335, 1995]. Microbiol Immunol 36: 1251-1275, 1992. PubMed: 1283774
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.