Specialty Cell Culture Media

  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030)

    ATCC® Number: PCS-600-030

    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $82.00 Non-Profit:   $82.00

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  • GeneXPlus Transfection Reagent (ATCC® ACS-4004)

    ATCC® Number: ACS-4004

    Quantity: 1 mL
    Storage: -20°C
    Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266). A complete list of cell line-specific protocols can be found here.

    For-Profit:   $357.00 Non-Profit:   $357.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4002) to reproducibly express a wide range of proteins at high levels.

  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011

    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.50 Non-Profit:   $25.50

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  • For-Profit:   $699.00 Non-Profit:   $699.00

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  • Stem Cell Dissociation Reagent (ATCC® ACS-3010)

    ATCC® Number: ACS-3010

    Quantity: 250 mg
    Storage: 2°C to 8°C

    For-Profit:   $92.00 Non-Profit:   $92.00

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

  • For-Profit:   $26.50 Non-Profit:   $26.50

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  • Stem Cell Freezing Media (ATCC® ACS-3020-3)

    ATCC® Number: ACS-3020-3

    Quantity: 3 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $128.00 Non-Profit:   $128.00

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    Product Format: frozen 3 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

    ATCC® Number: ACS-3002

    Quantity: 500 mL
    Storage: Complete medium is stored at -20°C

    For-Profit:   $235.00 Non-Profit:   $235.00

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    Product Format: frozen 500 mL
    Comments:
    This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.
  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020

    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $51.00 Non-Profit:   $51.00

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    Product Format: 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • ROCK Inhibitor Y27632 (ATCC® ACS-3030)

    ATCC® Number: ACS-3030

    Quantity: 10 mg
    Storage: -20°C to -80°C
    Applications:
    • Increased primary human organoid formation efficiency and recovery from thawing and passaging.
    • Enhanced survival and maintenance of pluripotency of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC)1.
    • Critical media component in conditional reprogramming of cells (CRC) culture technology2.

    For-Profit:   $199.00 Non-Profit:   $199.00

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    Product Format: dried 10 mg
    Comments:
    Purity is > 98% as confirmed by HPLC analysis. Structure and mass are confirmed by NMR and mass spectrometry.
    Reconstituted product can be aliquoted and stored at -20°C for up to six months. Protect from light.