Specialty Cell Culture Media

  • hTERT Immortalized RPTEC Growth Kit (ATCC® ACS-4007)

    ATCC® Number: ACS-4007

    Storage: -20°C
    Applications: Add to DMEM:F12 (ATCC® 30-2006™) to produce complete medium for hTERT Immortalized RPTEC cells (ATCC® CRL-4031™)

    For-Profit:   $240.00 Non-Profit:   $240.00

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    Product Format: frozen
    Comments: Each growth kit contains:
    PCS-999-058 5 ml RPTEC Supplement A
    PCS-999-059 10 ml RPTEC Supplement B

    Required but not supplied: G418 solution MUST be added to the above medium to a final concentration of 0.1 mg/mL G418 to maintain the selective pressure for immortalization 

    The hTERT immortalized RPTEC Growth Kit contains components that when added to DMEM:F12 (ATCC® 30-2006™) create a complete cell culture environment for hTERT immortalized RPTEC cells (ATCC® CRL-4031™). The final concentration for each growth kit component in the complete hTERT immortalized RPTEC growth medium is as follows:
    25 ng/mL Hydrocortisone
    3.5 µg/mL Ascorbic Acid
    8.65 ng/mL Sodium selenite
    5.0 µg/mL Transferrin
    5.0 µg/mL insulin
    5 pM Triiodo-L-thyronine
    25 ng/mL Prostaglandin E1
    10 ng/mL rhEGF
    1.2 µg/mL Sodium Bicarbonate 

    Note: Fetal Bovine Serum is not required for proliferation but may be added if it is desired to transfect the cells (final FBS concentration in complete medium is 0.2%).

  • Neural Progenitor Cell Dopaminergic Differentiation Kit (ATCC® ACS-3004)

    ATCC® Number: ACS-3004

    Applications:

    The media is specially designed for the dopaminergic differentiation of iPSC-derived neural progenitor cells (NPCs).

    Complete dopaminergic differentiation medium for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3004). To make complete NPC medium add the following components of this kit to 250 mL DMEM: F12:

    237 mL DMEM: F12 (Basal medium)
    2.5 mL L-Alanyl-L-Glutamine
    2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid
    5 mL Dopa Differentiation Kit Component A
    2.5 mLDopa Differentiation Kit Component B
    0.5 mL  Dopa Differentiation Kit Component C

    For-Profit:   $315.00 Non-Profit:   $315.00

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    Product Format: frozen
    Comments: 2.5 mL L-Alanyl-L-Glutamine
    2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid
    5 mL Dopa Differentiation Kit Component A
    2.5 mLDopa Differentiation Kit Component B
    0.5 mL Dopa Differentiation Kit Component C
  • Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003)

    ATCC® Number: ACS-3003

    Storage: -20°C
    Applications:

    The media is specially designed for expansion of iPSC-derived neural progenitor cells (NPCs).

    Complete growth media for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of this kit to 464 mL DMEM: F12:

    5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D

    For-Profit:   $310.00 Non-Profit:   $310.00

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    Product Format: frozen
    Comments: 5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D
    Focus Area:

    Neuronal Differentiation And Drug Screening

  • siFEX RNAi Transfection Reagent (ATCC® ACS-4006)

    ATCC® Number: ACS-4006

    Quantity: 500 µL
    Storage: Store at 2°C to 8°C
    Applications: Transient transfection, stable transfection, gene expression, siRNA transfection, miRNA transfection, gene knockdown.

    For-Profit:   $265.00 Non-Profit:   $265.00

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    Product Format: 500 µL
    Comments:

    siFEX RNAi Transfection Reagent is designed for transfection of small RNA sequences including siRNA and miRNA precursors into a broad array of mammalian cells with high efficiency and minimal toxicity. Cell line-specific protocols can be found here.

  • TransfeX™ Transfection Reagent (ATCC® ACS-4005)

    ATCC® Number: ACS-4005

    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

    For-Profit:   $230.00 Non-Profit:   $230.00

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality. Cell line-specific protocols can be found here.

  • Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC® PCS-500-041)

    ATCC® Number: PCS-500-041

    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)

    For-Profit:   $120.00 Non-Profit:   $120.00

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    Product Format: frozen 1 kit
    Comments:

    The Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs contains components that when added to Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human bone marrow. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:

    • rh FGF basic: 125 pg/mL
    • rh IGF-1: 15 ng/mL 
    • Fetal Bovine Serum: 7% 
    • L-Alanyl-L-Glutamine: 2.4 mM   
  • For-Profit:   $78.00 Non-Profit:   $78.00

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  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030)

    ATCC® Number: PCS-600-030

    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $82.00 Non-Profit:   $82.00

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  • GeneXPlus Transfection Reagent (ATCC® ACS-4004)

    ATCC® Number: ACS-4004

    Quantity: 1 mL
    Storage: -20°C
    Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266). A complete list of cell line-specific protocols can be found here.

    For-Profit:   $357.00 Non-Profit:   $357.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011

    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.50 Non-Profit:   $25.50

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