Specialty Cell Culture Media Page 1

GeneXPlus Transfection Reagent (ATCC^® ACS-4004^™)

ATCC^® Number: ACS-4004 ^™
Quantity: 1 mL
Storage: -20°C
Applications:

Transient transfection, stable transfection, gene expression, protein production, genetic manipulation. Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266).

For-Profit: $350.00 Non-Profit: $350.00

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1 mL

Comments:

This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCCACS-4500) and HEKPlus SFM (ATCCACS-4004) to reproducibly express a wide range of proteins at high levels.

Mammary Epithelial Cell Basal Medium (ATCC^® PCS-600-030^™)

ATCC^® Number: PCS-600-030 ^™
Quantity: 485 mL
Storage: 4 to 8°C, protect from light
Applications:

Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

Optional media supplements:

Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)

Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)

Phenol Red (ATCC PCS-999-001)

For-Profit: $80.00 Non-Profit: $80.00

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Product Format:

485 mL

VeroPlus SFM (ATCC^® ACS-4001^™)

ATCC^® Number: ACS-4001 ^™
Quantity: 500 mL
Storage: Store product at 2°C to 8°C. Do not freeze.

For-Profit: $40.00 Non-Profit: $40.00

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Product Format:

500 mL

Comments:

Each lot of VeroPlus SFM is tested functionally for growth characteristics and morphology with Vero cells. Additionally, the media is assayed for pH, osmolality, endotoxins and sterility.

Supplementing with L- Glutamine or L-alanyl-L-Glutamine is required before use.

TransfeX™ Transfection Reagent (ATCC^® ACS-4005^™)

ATCC^® Number: ACS-4005 ^™
Quantity: 500 μL
Storage: Store at 2°C to 8°C
Applications:

Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

For-Profit: $225.00 Non-Profit: $225.00

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Product Format:	500 μL
Comments:	Reagent is designed for transfection of primary cells and hard-to-transfect cell lines. TransfeX Transfection Reagent provides high efficiency with low cell mortality.

Mammary Epithelial Cell Growth Kit (ATCC^® PCS-600-040^™)

ATCC^® Number: PCS-600-040 ^™

For-Profit: $76.00 Non-Profit: $76.00

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frozen kit

Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs (ATCC^® PCS-500-053^™)

ATCC^® Number: PCS-500-053 ^™
Quantity: 1 kit
Storage: -20°C (or -70°C for long-term storage)
Applications: The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs contains medium and reagents designed to induce adipogenesis in actively proliferating Bone Marrow and Umbilical-Derived Mesenchymal Stem Cells with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:

One vial containing 1 mL of of ADM Supplement BMMSC/UCMSC for use in initiation of adipocyte differentiation

One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

* The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs provides enough medium and reagents for differentiation of ~6.8 x 10⁵ cells when plated at a recommended density of 18,000 viable cells/cm² in 4 wells of a 6 well tissue culture format.

For-Profit: $159.00 Non-Profit: $159.00

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Product Format:

frozen 1 kit