Specialty Cell Culture Media hTERT Immortalized RPTEC Growth Kit (ATCC® ACS-4007™) ATCC® Number: ACS-4007™ Storage: -20°C Applications: Add to DMEM:F12 (ATCC® 30-2006™) to produce complete medium for hTERT Immortalized RPTEC cells (ATCC® CRL-4031™) For-Profit: $240.00 Non-Profit: $240.00 Qty: Add to Cart Preview Product Compare Product Format: frozen Comments: Each growth kit contains: PCS-999-058 5 ml RPTEC Supplement A PCS-999-059 10 ml RPTEC Supplement B Required but not supplied: G418 solution MUST be added to the above medium to a final concentration of 0.1 mg/mL G418 to maintain the selective pressure for immortalization The hTERT immortalized RPTEC Growth Kit contains components that when added to DMEM:F12 (ATCC® 30-2006™) create a complete cell culture environment for hTERT immortalized RPTEC cells (ATCC® CRL-4031™). The final concentration for each growth kit component in the complete hTERT immortalized RPTEC growth medium is as follows: 25 ng/mL Hydrocortisone 3.5 µg/mL Ascorbic Acid 8.65 ng/mL Sodium selenite 5.0 µg/mL Transferrin 5.0 µg/mL insulin 5 pM Triiodo-L-thyronine 25 ng/mL Prostaglandin E1 10 ng/mL rhEGF 1.2 mg/mL Sodium Bicarbonate Note: Fetal Bovine Serum is not required for proliferation but may be added if it is desired to transfect the cells (final FBS concentration in complete medium is 0.2%). Product Sheet Certificateof Analysis SDS Neural Progenitor Cell Dopaminergic Differentiation Kit (ATCC® ACS-3004™) ATCC® Number: ACS-3004™ Applications: The media is specially designed for the dopaminergic differentiation of iPSC-derived neural progenitor cells (NPCs). Complete dopaminergic differentiation medium for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3004). To make complete NPC medium add the following components of this kit to 250 mL DMEM: F12: 237 mL DMEM: F12 (Basal medium) 2.5 mL L-Alanyl-L-Glutamine 2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid 5 mL Dopa Differentiation Kit Component A 2.5 mLDopa Differentiation Kit Component B 0.5 mL Dopa Differentiation Kit Component C For-Profit: $315.00 Non-Profit: $315.00 Qty: Add to Cart Preview Product Compare Product Format: frozen Comments: 2.5 mL L-Alanyl-L-Glutamine 2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid 5 mL Dopa Differentiation Kit Component A 2.5 mLDopa Differentiation Kit Component B 0.5 mL Dopa Differentiation Kit Component C Product Sheet Certificateof Analysis SDS Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003™) ATCC® Number: ACS-3003™ Storage: -20°C Applications: The media is specially designed for expansion of iPSC-derived neural progenitor cells (NPCs). Complete growth media for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of this kit to 464 mL DMEM: F12: 5 mL L-Alanyl-L-Glutamine 5 mL Non-Essential Amino Acids 10 mL NPC Growth Kit Component A 5 mL NPC Growth Kit Component B 1 mL NPC Growth Kit Component C 10 mL NPC Growth Kit Component D For-Profit: $310.00 Non-Profit: $310.00 Qty: Add to Cart Preview Product Compare Product Format: frozen Comments: 5 mL L-Alanyl-L-Glutamine5 mL Non-Essential Amino Acids10 mL NPC Growth Kit Component A5 mL NPC Growth Kit Component B1 mL NPC Growth Kit Component C10 mL NPC Growth Kit Component D Focus Area: Neuronal Differentiation And Drug Screening Product Sheet Certificateof Analysis SDS TransfeX™ Transfection Reagent (ATCC® ACS-4005™) ATCC® Number: ACS-4005™ Quantity: 500 μL Storage: Store at 2°C to 8°C Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation. For-Profit: $230.00 Non-Profit: $230.00 Qty: Add to Cart Preview Product Compare Product Format: 500 μL Comments: Reagent is designed for transfection of primary cells and hard-to-transfect cell lines. TransfeX Transfection Reagent provides high efficiency with low cell mortality. Cell line-specific protocols can be found here. Product Sheet Certificateof Analysis SDS Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC® PCS-500-041™) ATCC® Number: PCS-500-041™ Quantity: 1 kit Storage: -20°C (or -70°C for long-term storage) For-Profit: $120.00 Non-Profit: $120.00 Qty: Add to Cart Preview Product Compare Product Format: frozen 1 kit Comments: The Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs contains components that when added to Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human bone marrow. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows: rh FGF basic: 5 ng/mL rh IGF-1: 15 ng/mL Fetal Bovine Serum: 7% L-Alanyl-L-Glutamine: 2.4 mM Product Sheet Certificateof Analysis SDS Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030™) ATCC® Number: PCS-600-030™ Quantity: 485 mL Storage: 4 to 8°C, protect from light Applications: Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040). ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening. Optional media supplements: Gentamicin-Amphotericin B Solution (ATCC PCS-999-025) Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002) Phenol Red (ATCC PCS-999-001) For-Profit: $82.00 Non-Profit: $82.00 Qty: Add to Cart Preview Product Compare Product Format: 485 mL Product Sheet Certificateof Analysis SDS Mammary Epithelial Cell Growth Kit (ATCC® PCS-600-040™) ATCC® Number: PCS-600-040™ For-Profit: $78.00 Non-Profit: $78.00 Qty: Add to Cart Preview Product Compare Product Format: frozen kit Product Sheet Certificateof Analysis SDS Stem Cell Dissociation Reagent (ATCC® ACS-3010™) ATCC® Number: ACS-3010™ Quantity: 250 mg Storage: 2°C to 8°C For-Profit: $92.00 Non-Profit: $92.00 Qty: Add to Cart Preview Product Compare Product Format: 250 mg Comments: Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions. Product Sheet Certificateof Analysis SDS Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002™) ATCC® Number: ACS-3002™ Quantity: 500 mL Storage: Complete medium is stored at -20°C For-Profit: $235.00 Non-Profit: $235.00 Qty: Add to Cart Preview Product Compare Product Format: frozen 500 mL Comments: This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers. Product Sheet Certificateof Analysis SDS Stem Cell Freezing Media (ATCC® ACS-3020™) ATCC® Number: ACS-3020™ Quantity: 1 x 20 mL Storage: 2°C to 8°C For-Profit: $51.00 Non-Profit: $51.00 Qty: Add to Cart Preview Product Compare Product Format: 1 x 20 mL Comments: Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing. Product Sheet Certificateof Analysis SDS 1 2 >