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Specialty Cell Culture Media

  • hTERT Immortalized RPTEC Growth Kit (ATCC® ACS-4007™)

    ATCC® Number: ACS-4007™

    Storage: -20°C
    Applications: Add to DMEM:F12 (ATCC® 30-2006™) to produce complete medium for hTERT Immortalized RPTEC cells (ATCC® CRL-4031™)

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    Product Format: frozen
    Comments: Each growth kit contains:
    PCS-999-058 5 ml RPTEC Supplement A
    PCS-999-059 10 ml RPTEC Supplement B

    Required but not supplied: G418 solution MUST be added to the above medium to a final concentration of 0.1 mg/mL G418 to maintain the selective pressure for immortalization 

    The hTERT immortalized RPTEC Growth Kit contains components that when added to DMEM:F12 (ATCC® 30-2006™) create a complete cell culture environment for hTERT immortalized RPTEC cells (ATCC® CRL-4031™). The final concentration for each growth kit component in the complete hTERT immortalized RPTEC growth medium is as follows:
    25 ng/mL Hydrocortisone
    3.5 µg/mL Ascorbic Acid
    8.65 ng/mL Sodium selenite
    5.0 µg/mL Transferrin
    5.0 µg/mL insulin
    5 pM Triiodo-L-thyronine
    25 ng/mL Prostaglandin E1
    10 ng/mL rhEGF
    1.2 mg/mL Sodium Bicarbonate 

    Note: Fetal Bovine Serum is not required for proliferation but may be added if it is desired to transfect the cells (final FBS concentration in complete medium is 0.2%).

  • Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003™)

    ATCC® Number: ACS-3003™

    Storage: -20°C
    Applications:

    The media is specially designed for expansion of iPSC-derived neural progenitor cells (NPCs).

    Complete growth media for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of this kit to 464 mL DMEM: F12:

    5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D

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    Product Format: frozen
    Comments: 5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D
    Focus Area:

    Neuronal Differentiation And Drug Screening

  • Neural Progenitor Cell Dopaminergic Differentiation Kit (ATCC® ACS-3004™)

    ATCC® Number: ACS-3004™

    Applications:

    The media is specially designed for the dopaminergic differentiation of iPSC-derived neural progenitor cells (NPCs).

    Complete dopaminergic differentiation medium for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3004). To make complete NPC medium add the following components of this kit to 250 mL DMEM: F12:

    237 mL DMEM: F12 (Basal medium)
    2.5 mL L-Alanyl-L-Glutamine
    2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid
    5 mL Dopa Differentiation Kit Component A
    2.5 mLDopa Differentiation Kit Component B
    0.5 mL  Dopa Differentiation Kit Component C

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    Product Format: frozen
    Comments: 2.5 mL L-Alanyl-L-Glutamine
    2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid
    5 mL Dopa Differentiation Kit Component A
    2.5 mLDopa Differentiation Kit Component B
    0.5 mL Dopa Differentiation Kit Component C

  • TransfeX™ Transfection Reagent (ATCC® ACS-4005™)

    ATCC® Number: ACS-4005™

    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality. Cell line-specific protocols can be found here.

  • Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC® PCS-500-041™)

    ATCC® Number: PCS-500-041™

    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)

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    Product Format: frozen 1 kit
    Comments:

    The Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs contains components that when added to Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human bone marrow. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:

    • rh FGF basic: 5 ng/mL
    • rh IGF-1: 15 ng/mL 
    • Fetal Bovine Serum: 7% 
    • L-Alanyl-L-Glutamine: 2.4 mM   

  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030™)

    ATCC® Number: PCS-600-030™

    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

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    Product Format: 485 mL

  • Mammary Epithelial Cell Growth Kit (ATCC® PCS-600-040™)

    ATCC® Number: PCS-600-040™

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    Product Format: frozen kit

  • Stem Cell Freezing Media (ATCC® ACS-3020™)

    ATCC® Number: ACS-3020™

    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.

  • Stem Cell Dissociation Reagent (ATCC® ACS-3010™)

    ATCC® Number: ACS-3010™

    Quantity: 250 mg
    Storage: 2°C to 8°C

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002™)

    ATCC® Number: ACS-3002™

    Quantity: 500 mL
    Storage: Complete medium is stored at -20°C

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    Product Format: frozen 500 mL
    Comments:
    This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.