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THP-1 Reporter Cells

Gray green and pink bunch of dendric T-cells.

Quantification of immunomodulation made easy

The lack of stable and sensitive advanced immunology cell-based models to evaluate immune activation has hindered immuno-oncology research and development for decades. To address this need, ATCC introduced luciferase reporters containing the response element of immunologically important transcription factors into the THP-1 cell line.

The THP-1 LUC2 cell lines provide a means to confidently measure immune modulation for all your drug discovery and development efforts. Originating from a spontaneously immortalized human monocyte-like cell line that naturally expresses many pattern-recognition and cytokine receptors, ATCC THP-1 LUC2 cells represent the most  physiologically relevant model to aid advancements in immuno-oncology and immune disorders.

Features and Benefits of THP-1 Reporter Cells

Key features Key benefits 
Fully authenticated parental THP-1 cell line  No concerns about cross-contamination and misidentification, saves time and money 
High signal-to-noise ratio Clear and more intense results, straightforward data analysis
Verified, characterized stable expression Reduced variability, reproducible results
Easy to culture, robust, and highly sensitive Ease of use, customer convenience
Amenable to complex experimentation (combinatorial stimulation, co-culture) Versatile and compatible with multiple platforms
High density cryopreservation More viable cells post-thaw


Quantitation of immunomodulation made easy

Quantitation of immunomodulation made easy. To use THP-1 LUC2 cells, simply seed in a 96-well plate. Stimulate the cells overnight with your compound of interest, then incubate the cells using a luciferase assay system and read the bioluminescence signals using a luminometer. Your immunomodulation data will be bigger, brighter, better.


Available THP-1 LUC2 reporter cell lines

Response element ATCC No. Signaling Pathway  Function 
NFκB TIB-202-NFκB-LUC2 NFκB Pivotal mediator of inflammatory response
GAS  TIB-202-GAS-LUC2  JAK-STAT (Type II) Initiates immune cell activation and recruitment
CRE TIB-202-CRE-LUC2  cAMP/PKA Inflammatory mediator and phagocytosis modulator
ISRE  TIB-202-ISRE-LUC2  JAK-STAT (Type I) Initiates immune cell activation and recruitment
AP1  TIB-202-AP1-LUC2  MAPK/ERK Regulates innate and adaptive immune response
NFAT  TIB-202-NFAT-LUC2  Calcineurin-NFAT Mediates adaptive T and B cell activation


These high-quality cell lines are well suited to study the role of proteins involved in signaling cascades activated by immunomodulators, to optimize the MoA, pharmaceutical potency, and/or toxicological profile of leading drug candidates, and to evaluate the efficacy or toxicity of promising drug compounds in vitro assays.


Comparison of luminescence and in vitro quantification of luciferase activity of THP-1 LUC2 and competitor reporter cell  lines.


Comparison of luminescence and in vitro quantification of luciferase activity of THP-1 LUC2 and competitor reporter cell lines. Cells were seeded in a 96-well plate. After overnight stimulation with the appropriate interferons, bioluminescence signals were detected using a commercially available luciferase assay kit and a luminometer. Error bars show standard deviation (n=3). (A) ATCC THP-1 GAS-Luc2 (orange bar), THP-1 ISRE-Luc2 (yellow bar), or competitor immune regulator expression cells (green bar) stimulated with the indicated interferons and assessed for bioluminescence. (B) ATCC THP-1 NFkB-Luc2 (orange bar) or competitor immune regulator expression cells (green bar) treated with the indicated toll-like receptor agonists and assessed for bioluminescence intensity. In both studies, THP-1 luciferase-expressing cells exhibited enhanced bioluminescence signal compared to the competitor cells.

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