Escherichia coli (Migula) Castellani and Chalmers (ATCC® CRM-11229)

Strain Designations: AMC 198  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

View Permits

Classification Enterobacteriaceae, Escherichia
Strain Designations AMC 198
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009
Bacterial resistance testing paint
Membrane filter testing
Testing absorbent pads
Testing antimicrobial handwashing formulations
Testing disinfectants
Testing ink grids
Testing sanitizers
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Intended Use



Certified Reference Material produced under an ISO Guide 34:2009 accredited process.


    ACLASS Accredited Reference Material Producer AR-1384

Preceptrol® no
Type Strain no
Comments Certificates of Analysis are available electronically at, or by hardcopy upon request.


Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor AMC - Walter Reed Army Medical Center

ASTM International Standard Test Method for Evaluation of Health Care Personnel Handwash Formulation. West Conshohocken, PA

ASTM International Standard Test Method for Evaluation of Antimicrobial Handwash Formulations by Utilizing Fingernail Regions. West Conshohocken, PA

ASTM International Standard Methods for Evaluating Absorbent Pads Used with Membrane Filters for Bacteriological Analysis and Growth. West Conshohocken, PA (Reapproved 2003).

AOAC International Disinfectants (water) for swimming pools. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 965.13.

Machemer WE. Use of mercury in protective coatings. Dev. Ind. Microbiol. 20: 25-39, 1979.

Confimatory Efficacy Data Requirements. Washington, DC:Environmental Protection Agency;EPA EPA DIS/TSS-5, 1982

Standard Test Method for Determining the Bacteria-Eliminating Effectiveness of Hygienic Handwash and Handrub Agents using the Fingerpads of Adult Subjects. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2276-03E01.

Textile fabrics --- Determination of antibacterial activity --- Agar diffusion plate test. London, UK:British Standards Institution;British Standard BS EN ISO 20645:2004.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation