Candida utilis (Henneberg) Lodder et Kreger-van Rij (ATCC® 9950)

Alternate State: Cyberlindnera jadinii (Sartory et al.) Minter, teleomorph  /  Strain Designations: NRRL Y-900 [CBS 5609, CCRC 20325, DSM 2361, IFO 0988, NBRC 0988, NCYC 707, NRCC 2721, VTT C-78085]  /  Product Format: freeze-dried

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Deposited As Torulopsis utilis (Henneberg) Lodder
Strain Designations NRRL Y-900 [CBS 5609, CCRC 20325, DSM 2361, IFO 0988, NBRC 0988, NCYC 707, NRCC 2721, VTT C-78085]
Alternate State Cyberlindnera jadinii (Sartory et al.) Minter, teleomorph
Application
Accumulates zinc
Assay of antimicrobial agent
Degrades RNA ribonucleic acid
Degrades alfalfa process wastes
Degrades ethyl alcohol ethanol
Degrades pickle process brine
Degrades potato wastes
Degrades rapeseed oil meal
Degrades sauerkraut brine
Produces S-adenosylmethionine
Produces acetaldehyde
Produces ethyl acetate
Produces methionine adenosyltransferase S-adenosylmethionine synthetase
Produces single-cell protein SCP
Quality control strain
Transformation host
Transformation host for expression of carotenogenic genes
Used in Controlled Ecological Life Support Systems (CELSS) for food
Produces adenosyl-D-methionine and adenosyl-2-methylmethionine
Produces high quality edible protein
Conversion of 5'-methylthioadenosine into S-adenosylmethionine
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain

Yes

Comments
Cell vacuoles
Genome sequencing strain (KIRIN Holdings Company, Japan)
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions
Temperature: 25°C to 30°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GCGCTTCTAGCGCGGCTCCACCAATACACAGTGTATTTTGCTTCTTTTGCTTTGGCTCTGCCAAAGGTTTTTAAACACAGAAATTTATTTTCTCTAGAAACTAGTCAATTTGAATTTTAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCAACGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGGTTTTCGTGAATCATCGAATCTTTGAACGCATATTGCGCTCTCTGGCATTCCAGAGAGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAGATCCTCTAGGGGACTTGGTATTGAGTGATACTCTGTGTTAACTTGAAATACTCTAGGCAGAGCTCCCCCTAGAAATCCTCTGGGCCGAAATAATGTATTAGGTTCTACCAACTCGTTATTTTCCAGACAGACTTCCAGGCAGAGCTCGGC


D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGAGGCTYTCAGCCCCCGAGTTGTAATTTGAAGATGGTGTTCTGGTGCCGGCCCCCTGTCTACGTTCCTTGGAACAGGACATCACAGAGGGTGAGAATCCCGTCTGGCGGGGCGGCCTGGCTCCGTGTAGAGCGCCATCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTGGATCAGACTTGGTGCTGTGCGAATAGCGGCYCTTCTTGGGCYGCCCACTCGCACTCCACCGGGCCAGCATCGGTTTGGGCGGCAAGACAATGGCGGSGGAACGTGGCACTGCTCTCGGGCAGTGTGTTTATAGCCCCCGCTGATGTTGCCTGCCTAGACCGAGGACTGCGGCTTCTGCCTAGGATGCTGGCGTAATGATCCAACACCGC

Name of Depositor NRRL
Cross References

Nucleotide (GenBank) : AJ277098 Candida utilis anc gene for mitochondrial ADP/ATP carrier, isoform

Nucleotide (GenBank) : AJ277099 Candida utilis anc gene for mitochondrial ADP/ATP carrier, isoform

References

Tamakawa H, Ikushima S, Yoshida S. Construction of a Candida utilis strain with ratio-optimized expression of xylose-metabolizing enzyme genes by cocktail multicopy integration method. J Biosci Bioeng 115: 532-539, 2013. PubMed: 23294574

Tamakawa H, et al. Metabolomic and transcriptomic analysis for rate-limiting metabolic steps in xylose utilization by recombinant Candida utilis. Biosci Biotechnol Biochem 77: 1441-1448, 2013. PubMed: 23832335

Tomita Y, et al. Genome and transcriptome analysis of the food-yeast Candida utilis. PLoS One 7: e37226, 2012. PubMed: 22629373

Suzuki T, et al. Identification and characterization of genes involved in glutathione production in yeast. J Biosci Bioeng 112: 107-113, 2011. PubMed: 21601516

Ostermann K, et al. Identification of the genes GPD1 and GPD2 of Pichia jadinii. DNA Seq 17: 452-457, 2006. PubMed: 17381046

Park YC, et al. Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950. J Ind Microbiol Biotechnol 33: 1032-1036, 2006. PubMed: 16855819

Miura Y, et al. Production of the carotenoids lycopene, beta-carotene, and astaxanthin in the food yeast Candida utilis. Appl. Environ. Microbiol. 64: 1226-1229, 1998. PubMed: 9546156

Kondo K, et al. A transformation system for the yeast Candida utilis: use of a modified endogenous ribosomal protein gene as a drug-resistant marker and ribosomal DNA as an integration target for vector DNA. J. Bacteriol. 177: 7171-7177, 1995. PubMed: 8522525

Peterson GR. Reproducible analyses of microbial food for advanced life support systems. Enzyme Microb. Technol. 10: 586-592, 1988.

Armstrong DW, et al. Production of ethyl acetate from dilute ethanol solutions by Candida utilis. Biotechnol. Bioeng. 26: 1038-1041, 1984.

Armstrong DW, et al. Production of acetaldehyde from ethanol by Candida utilis. Biotechnol. Lett. 6: 183-188, 1984.

Lawford HG, et al. Hyperaccumulation of zinc-depleted Candida utilis grown in chemostat culture. Can. J. Microbiol. 26: 71-76, 1980. PubMed: 7190863

Mudgett RE, et al. Single cell protein recovery from alfalfa process wastes. Trans. ASAE 23: 1590-1594, 1980.

Shapiro SK, Schlenk F. Conversion of 5'-methylthioadenosine into S-adenosylmethionine by yeast cells. Biochim. Biophys. Acta 633: 176-180, 1980. PubMed: 7193054

Lawford GR, et al. Production of high-quality edible protein from Candida yeast grown in continuous culture. Biotechnol. Bioeng. 21: 1163-1174, 1979.

Phillipchuk GE, Jackson H. Rapeseed oil meal as a nitrogenous substrate for microbial fermentation. J. Gen. Appl. Microbiol. 25: 117-125, 1979.

Stevenson KE, et al. Aerobic fermentations of pickle process brine by Candida utilis. J. Food Sci. 44: 181-185, 1979.

Schlenk F, et al. Biosynthesis of adenosyl-D-methionine and adenosyl-2-methylmethionine by Candida utilis. Arch. Biochem. Biophys. 187: 191-196, 1978. PubMed: 566086

Moreton RS. Growth of Candida utilis on enzymatically hydrolyzed potato waste. J. Appl. Bacteriol. 44: 373-382, 1978.

Riviere J, et al. Production de proteines microbiennes a partir de mouture de ble. Ann. Technol. Agric. 27: 585-607, 1978.

Irgens RL, Clarke JD. Production of single-cell protein by the cultivation of yeast in anaerobic digester supernatant supplemented with carbohydrates. Eur. J. Appl. Microbiol. 2: 231-241, 1976.

Holcomb ER, Shapiro SK. Assay and regulation of S-adenosylmethionine synthetase in Saccharomyces cerevisiae and Candida utilis. J. Bacteriol. 121: 267-271, 1975. PubMed: 1090572

Brewer D, et al. The antibiotic activity of cultures from fungal spores collected by a spore trap on permanent pasture. Can. J. Microbiol. 20: 721-729, 1974. PubMed: 4857622

Nakamura KD, Schlenk F. Examination of isolated yeast cell vacuoles for active transport. J. Bacteriol. 118: 314-316, 1974. PubMed: 4362463

Hang YD, et al. Sauerkraut waste: a favorable medium for cultivating yeasts. Appl. Microbiol. 24: 1007-1008, 1972.

. . Trans. ASAE 14: 103-122, 1972.

Ohta S, et al. Characterization of a heat-shock process for reduction of the nucleic acid content of Candida utilis. Appl. Microbiol. 22: 415-421, 1971. PubMed: 4330316

Maul SB, et al. New process for reducing the nucleic acid content of yeast. Nature 228: 181, 1970. PubMed: 5460024

Reiser CO. Torula yeast from potato starch wastes. J. Agric. Food Chem. 2: 70-74, 1954.

VITEK 2 YST Comprehensive QC Set. bioMerieux.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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