pENTR_L1L2_DONR1 (ATCC® SB-1013)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L1L2_DONR1
Depositors R Weiss, Massachusetts Institute of Technology
Applications Empty entry vector from the Gateway® system that is designed to accept any gene from the ATCC® Synthetic Biology Yeast Tool kit.

Gateway® is a registered trademark of Thermo Fisher Scientific.

Vector Total size of construct (kb): 3.266
Vector type: Donor vector
Markers: kanR
Insert Insert size (kb): 0.67
Suggested primer for sequencing:
5’- GTCCTACTCAGGAGAGC - 3’
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1014
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The vector contains a LacZ alpha gene flanked by two BsaI sites that is inserted between two att sites (att L1 and att L2) for a Golden Gate-compatible cloning step to insert any gene of interest.
References

Life Technologies™. Gateway® Technology User Manual. September 2003.