pDEST23y (ATCC® SB-1001)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pDEST23y
Depositors R Weiss, Massachusetts Institute of Technology
Applications A destination vector in the ATCC® Synthetic Biology Yeast Tool Kit designed for use in the development of a single transcription unit
Vector Total size of construct (kb): 6.419
Vector type: Destination vector
Markers: ampR and cmR
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1000
ATCC SB-1002
ATCC SB-1003
ATCC SB-1015
ATCC SB-1016
ATCC SB-1017
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
This plasmid is a Saccharomyces cerevisiae/Escherichia coli shuttle vector. It contains a HIS3 marker for yeast selection. A ccdB gene, located within two att sites, can improve the Gateway® cloning efficiency.
Gateway® is a registered trademark of Thermo Fisher Scientific
References

Life Technologies™. Gateway® Technology User Manual. September 2003.