YUMM1.G1 (ATCC® CRL-3363)

Organism: Mus musculus, mouse  /  Tissue: skin  /  Disease: melanoma

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Organism Mus musculus, mouse
Tissue skin
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease melanoma
Gender female
Strain c57bl/6j mcr1(e/e)
Applications Mouse model of melanoma and interactions with the immune microenvironment. Contains human-relevant mutations. Subcutaneous injections can form tumors in C57BL/6J mice
Storage Conditions liquid nitrogen vapor phase
Images Cell Micrograph of YUMM1.G1 cells, ATCC CRL-3363
Oncogene BrafV600E/wt (knock in conditionally activated Braf allele)
Cdkn2a-/- Pten-/- (conditionally inactivated)
Comments Tumor derived from c57bl/6j mcr1(e/e) - extension locus; blonde hair color. This cell line is genetically modified.
Tyrosinase:CreERT2 transgene restricts recombination to melanocytes.
BrafV600E/wt (knock in conditionally activated Braf allele)
Cdkn2a-/- Pten-/- (conditionally inactivated)
Complete Growth Medium The base medium for this cell line is DMEM: F12 (ATCC 30-2006). To make the complete medium add the following components to the base medium:
  • 56 mL fetal bovine serum (FBS) (ATCC 30-2020)
  • 5.6 mL NEAA (Gibco Cat# 11440-076)
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 2 x 104 and 4 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 8 X 103 and 6.0 X 105 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Note: Subculture at confluence ≤90% as cells tend to lift off in sheets at higher confluence
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Approximately 1.5 to 2.5 x 106 cells
Volume 1.0 mL
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viability ≥ 50%
Name of Depositor M Bosenberg. Yale University School of Medicine, Dermatology
Year of Origin 2014
References

Meeth K, et al. The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597, 2016. PubMed: 27287723

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Meeth K, et al. The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597, 2016. PubMed: 27287723