FOX-NY (ATCC® CRL-1732)

Organism: Mus musculus, mouse  /  Cell Type: B lymphoblast  /  Disease: myeloma

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease myeloma
Strain BALB/c
Applications
FOX-NY was derived from the NS-1 myeloma cell line.
It is resistant to analogs of both adenosine and guanine (e.
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
Selection for the Igh locus results in more stable antibody production in the resulting hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
FOX-NY was derived from the NS-1 myeloma cell line.
Clinical Data
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
Comments
FOX-NY was derived from the NS-1 myeloma cell line.
It is resistant to analogs of both adenosine and guanine (e.g., 2,6-diaminopurine and 8-azaguanine) as a result of deficiencies in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and adenosine phosphoribosyl transferase (APRT).
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
If the fusion products are grown in the presence of aminopterin, adenine and thymidine (or azaserine and adenine).
Selection for the Igh locus results in more stable antibody production in the resulting hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor RT Taggart
Deposited As Mus musculus
References

Taggart RT, Samloff IM. Stable antibody-producing murine hybridomas. Science 219: 1228-1230, 1983. PubMed: 6402815

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Taggart RT, Samloff IM. Stable antibody-producing murine hybridomas. Science 219: 1228-1230, 1983. PubMed: 6402815