CCD-1065Sk (ATCC® CRL-2077)

Organism: Homo sapiens, human  /  Cell Type:: fibroblast  /  Disease: Adenocarcinoma

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Adenocarcinoma
Age 46 years
Gender female
Ethnicity Black
Applications
The line was established from skin taken from normal breast tissue removed at mastectomy for metastatic adenocarcinoma.
Derivation
The line was established from skin taken from normal breast tissue removed at mastectomy for metastatic adenocarcinoma.
Clinical Data
female
Black
46 years
Comments
The line was established from skin taken from normal breast tissue removed at mastectomy for metastatic adenocarcinoma.
The cells senesce after approximately 33 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%