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LASCPC-01

CRL-3356

LASCPC-01 is a cell line exhibiting aggregate morphology that was isolated in 2014 from the prostate of a 75 to 80-year old, White male patient with prostate cancer. This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of neuroendocrine prostate cancer (NEPC).
Product category
Human cells
Organism
Homo sapiens, human
Morphology
aggregate
Tissue
Prostate
Disease
Prostate Cancer
Applications
3D cell culture
Cancer research
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Cells contain Lentivirus genes

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of Neuroendocrine prostate cancer (NEPC)

Characteristics

Volume
1.0 mL
Growth properties
Mixed: suspension and aggregate
Age
75 to 80 years
Ethnicity
White
Gender
Male
Karyotype
Conventional karyotyping: normal 46 X,Y
Array CGH: mosaic gian of 1q23.1-1q44
Comments
Expression of RFP, GFP, MYCN, and myristoylated AKT1.

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium

To prepare 500mL of complete medium:

  • 464 mL Modified HITES medium (prepared as below)
  • 1 mL Hydrocortisone (Sigma cat# H-0135; 10 nM or 1.0 x 10-8 M)
  • 5 mL Transferrin (Sigma cat# T-5391; 0.01 mg/mL or 10 µg/mL)
  • 25 mL FBS (ATCC 30-2020)
  • 5 mL L-Glutamine (ATCC 30-2214)
Modified HITES Medium
  • Insulin, human recombinant (Life Technologies cat# 12585-014), 50 mg 
  • Sodium Selenite (Sigma cat# S9133), 0.04326 mg 
  • Β-Estradiol (Sigma cat# E2257), 0.02724 mg 
  • L-Glutamine (ATCC 30-2214), 2.922 g 
  • RPMI-1640 (ATCC 30-2001), 10 L
Adjust pH to 7.2 – 7.4. Filter sterilize through a 0.2 micrometer filter, dispense 500 mL into 500 mL bottles.

Note: Complete culture medium is stable for 1 month once hydrocortisone and transferrin are added.

Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 150-400 x g for 8 to 12 minutes.
  4. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
  6. Subculture when there are numerous, healthy appearing clusters present in suspension
Subculturing procedure
Cultures can be maintained by addition of fresh medium once or twice a week.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Population doubling time
Approximately 24 hrs
STR profiling
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 9
TPOX: 8,11
vWA: 15,17
Viability
≥ 50%

History

Depositors
J Lee
Year of origin
2014

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

For-profit Research Use License from University of California, Los Angeles

For-profit Organizations: For every order of this item, you must work directly with the contributor, University of California, Los Angeles to (i) negotiate a research-use license, and/or (ii) have the contributor provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from University of California, Los Angeles that we are authorized to ship each order.

We are providing the following contact information, but this information may change without notice:
UCLA Technology Development Group
Phone: 310-794-0558
Email: [email protected]

Once ATCC has received authorization from University of California, Los Angeles, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Lee JK, et al. N-Myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Canc Cell 29(4):536-47, 2016. PubMed: 27050099

Need assistance with this product? Contact our Technical Support team.

Telephone

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800-638-6597

Outside the US
+1-703-365-2700

Hours of Operation

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