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Dengue virus type 1 (ATCC® VR-1856)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 1.0 mL per vial

Classification Flaviviridae, Flavivirus
Agent Dengue virus type 1
Strain Hawaii
Applications Vector-borne disease research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1856 images
Comments Derived by adaptation of mouse-prepared product ATCC® VR-1254™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1254™ and ATCC® VR-1856™ have not been compared for sequence or infectivity in tissue culture and in vivo.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.02). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 7-15 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor R Selvarangan, Children’s Mercy Hospital
Source Pooled serum from 6 patients, Hawaii, 1944
References

Sabin AB. . Science 101: 640, 1945.

Sabin AB. Viral and Rickettsial Infections of Man. Philadelphia: JB Lippincott Co; 1959.