Human herpesvirus 6B (ATCC® VR-1467)

Classification: Herpesviridae, Roseolovirus  /  Product Format: frozen

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Classification Herpesviridae, Roseolovirus
Agent Human herpesvirus 6B
Strain Z-29
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Vapor of liquid nitrogen
Comments
Causes exanthum subitum (roseola infantum)
Implicated in a number of childhood syndromes
Will not survive long-term storage at -70°C
This variant is adapted for growth on Molt-3 cells
Effect on Host
Syncytia and enlarged single cells
Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Recommended Host
MOLT-3, human T cell line (ATCC® CRL-1552™)
Growth Conditions

Incubation: 7 days at 37°C, a 5% CO2 in air atmosphere is recommended. Multiple 7 day passes may be required to achieve acceptable titer. It may be difficult to obtain high titer preparations. 

Recommendations for Infection: For best results, initial inoculation should proceed directly after splitting of the uninfected MOLT 3 cells.

Effect on Host
Syncytia and enlarged single cells
Name of Depositor JB Black, CDC
Special Collection NCRR Contract
Source Peripheral blood lymphocytes from a 36 year-old male AIDS patient, Zaire, Africa
References

Lopez C, et al. Characteristics of human herpesvirus-6. J. Infect. Dis. 157: 1271-1273, 1988. PubMed: 3259614

Black JB, et al. Growth properties of human herpesvirus-6 strain Z29. J. Virol. Methods 26: 133-145, 1989. PubMed: 2693479

can also be grown on human T cells

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Basic Documentation