Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human (ATCC® PCS-800-011)

Organism: Homo sapiens, human  /  Tissue: Blood  /  Cell Type: Mononuclear

Permits and Restrictions

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Organism Homo sapiens, human
Tissue Blood
Cell Type Mononuclear
Morphology spherical; variable after culturing
Growth Properties suspension; variable after culturing
Biosafety Level 1

 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age lot specific
Gender lot specific
Ethnicity lot specific
Applications Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.
Product Format frozen 1 mL
Storage Conditions -130°C
Images
Comments Peripheral blood mononuclear cells (PBMC) have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors. 

Peripheral blood mononuclear cells are a heterogeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes and lymphocytes.

Complete Growth Medium Peripheral blood mononuclear cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.

Note: Application specific medium, cytokines and other factors

Subculturing N/A
Volume 1 mL
Cells per Vial One vial contains a minimum of 2.5 x 107 viable cells.
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV(I/II): Negative
HTLV(I/II): Negative
WNV: Negative
Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
Population Doubling Capacity N/A
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Aboul‐Enein M N, et al. Design, Synthesis, and Cytotoxic Evaluation of Certain 7‐Chloro‐4‐(piperazin‐1‐yl) quinoline Derivatives as VEGFR‐II Inhibitors. Arch Pharm Weinheim 350: 3-4, 2017. PubMed: 28304102

Hou W, et al. Determination of the Cell Permissiveness Spectrum, Mode of RNA Replication, and RNA-Protein Interaction of Zika Virus. BMC Infect Disease, 17(1):239, 2017. PubMed: 28359304

Mohamed, M, et al. Cytotoxicity And Anti-Cancer Effect Of Mangrove Crab (Scylla Serrata) Soup On Human Leukemic Jurkat Cells. Eur J Pharma Med Res 4(2): 192-195, 2017.

Li, X et al. Brain Natriuretic Peptide-Regulated Expression of Inflammatory Cytokines in Lipopolysaccharide (LPS)-Activated Macrophages Via NF-kB and Mitogen Activated Protein Kinase (MAPK) Pathways. Med Sci Monit, 24: 3119-3126, 2018. PubMed: 29754152

Kim Wiese, A, et al. DNA-PKcs Controls Calcineurin Mediated IL-2 Production in T Lymphocytes. PloS One, 12(7):e0181608, 2017. PubMed: 28750002

Ali SA, et al. A Cell Internalizing Antibody Targeting Capsid Protein (p24) Inhibits the Replication of HIV-1 in T Cells Lines and PBMCs: A Proof of Concept Study. PloS One, 11(1):e0145986, 2016. PubMed: 26741963

Farhang N, et al. CRISPR-Based Epigenome Editing of Cytokine Receptors for the Promotion of Cell Survival and Tissue Deposition in Inflammatory Environments. Tissue Eng Part A, 23(15-16):738-749, 2017. PubMed: 28095751

Nicolae CM, et al. NFkB regulates p21 expression and controls DNA damage-induced leukemic differentiation. Oncogene doi: 10.1038/s41388-018-0219-y, 2017. PubMed: 29622796

Marunde M. Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA Technology. PerkinElmer, 2017.

Cui, X, et al. A Fluorescent Microbead-based Microfluidic Immunoassay Chip for Immune Cell Cytokine Secretion Quantification. Lab Chip 18(3):522-531, 2018. PubMed: 29326990