Caki-1 (ATCC® HTB-46)

Organism: Homo sapiens, human  /  Tissue: kidney:skin  /  Disease: clear cell carcinoma

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Organism Homo sapiens, human
Tissue
kidney:skin
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease clear cell carcinoma
Age 49 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 68; range = 63 to 71.
The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
Derivation
­
Clinical Data
49 years
Caucasian
male
Antigen Expression
Antigen expression: Blood Type O; Rh-; HLA A9, B12, Bw35
Genes Expressed
Tumorigenic Yes
Effects
Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters
Comments
Ultrastructural features include many microvilli, few filaments, many small mitochondria, well developed Golgi and ER, many lipid droplets and multilaminate bodies, secondary lysosomes, no virus particles.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,11
    D13S317: 11,12
    D16S539: 12
    D5S818: 11,12
    D7S820: 8,12
    THO1: 6,8
    TPOX: 8,11
    vWA: 15,17
    Isoenzymes
    AK-1, 1
    ES-D, 1-2
    G6PD, B
    GLO-I, 1-2
    Me-2, 2
    PGM1, 1
    PGM3, 1-2
    Name of Depositor J Fogh
    References

    Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

    Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

    Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

    Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

    Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Restrictions

    The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

    References

    Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

    Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

    Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

    Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

    Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047