SU-DHL-16 (ATCC® CRL-2964)

Organism: Homo sapiens, human  /  Tissue: lymph node
Derived from Metastatic Site: peritoneal effusion

 /  Disease: large cell lymphoma; B cell lymphoma

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Organism Homo sapiens, human
Tissue lymph node
Derived from Metastatic Site: peritoneal effusion

Morphology lymphoblast-like
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease large cell lymphoma; B cell lymphoma
Storage Conditions liquid nitrogen vapor phase
Karyotype t(14;18) chromosomal translocation, positive
Images CRL-2964 Micrograph
Genes Expressed methionine aminopeptidase 2 (MetAP2), expressed
cathelicidin antimicrobial peptide (CAMP) expression is induced upon exposure to 1,25(OH)2D3
bcl-6 protein, expressed
Comments The depositor states that this cell line is negative for the presence of Epstein-Barr virus(EBV) genome.
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
The SU-DHL-16 cell line possesses a t(14;18)(q32;q21) translocation.
The SU-DHL-16 cell line contains a minor bcl-2 rearrangement.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 2 x 105 cells/mL is recommended. Subculture when cell concentration is between 1 x 106 and 2 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 10
D13S317: 9, 12
D7S820: 9, 10
D16S539: 11, 13
vWA: 15, 18
THO1: 7, 9.3
TPOX: 8, 11
CSF1PO: 10, 11
Amelogenin: XY

Name of Depositor A Epstein
Year of Origin 1980
References

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039