Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47024)

Strain Designations: BNN93 [C600]  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations BNN93 [C600]
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Shipping Information

Shipped:  Frozen glycerol stock of Escherichia coli.

Storage Conditions

Conditions: Store unopened frozen vial at -80°C.

Preceptrol® no
Genotype F- hsdR supE44 thr-1 leuB6 thi-1 lacY1 fhuA21 e14- mcrB lambda-
Type Strain no
Comments
A general purpose host strain suitable for lambdagt10.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- hsdR supE44 thr-1 leuB6 thi-1 lacY1 fhuA21 e14- mcrB lambda-
Name of Depositor R Young, RW Davis
References

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985

Raleigh EA, et al. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 16: 1563-1575, 1988. PubMed: 2831502

Woodcock DM, et al. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17: 3469-3478, 1989. PubMed: 2657660