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Methanothermobacter wolfeii (Winter et al.) Wasserfallen et al. (ATCC® 43096)

Strain Designations: DSM 2970  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Methanobacterium wolfei Winter et al.
Strain Designations DSM 2970
Produces restriction endonuclease MwoI
Sewage sludge and river sediment mixture
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Medium ATCC® Medium 1340: MS medium for methanogens
Growth Conditions
Temperature: 60°C
Atmosphere: Anaerobic gas mixture, 80% H2-20% CO2
Name of Depositor J Winter
Sewage sludge and river sediment mixture

Validation list no. 17. Int. J. Syst. Bacteriol. 35: 223-225, 1985.

Winter J, et al. Methanobacterium wolfei, sp.nov., a new tungsten-requiring, thermophilic, autotrophic methanogen. Syst. Appl. Microbiol. 5: 457-466, 1984.

Lunnen KD, et al. Characterization and cloning of MwoI(GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei. Gene 77: 11-19, 1989. PubMed: 2663652

Wasserfallen A, et al. Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. Int. J. Syst. Evol. Microbiol. 50: 43-53, 2000. PubMed: 10826786

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation