Treponema denticola (ex Flügge) Chan et al. (ATCC® 35405)

Strain Designations: a [CIP 103919, DSM 14222]  /  Type Strain: yes  /  Biosafety Level: 1

Deposited As Treponema denticola (Brumpt) Chen
Strain Designations a [CIP 103919, DSM 14222]
Application
Produces major surface protein
Produces methyl-accepting chemotaxis protein
Produces prolyl aminopeptidase proline iminopeptidase
Isolation
Human periodontal pocket, Montreal, Canada
Type Strain yes
Biosafety Level 1
Antigenic Properties serovar a
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Treponema denticola ATCC 35405, complete genome.

Type Strain yes
Antigenic Properties serovar a
Comments
Benzoylarginine peptidase and iminopeptidase profiles
Genome sequenced strain
Medium ATCC® Medium 1494: Modified NOS medium
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor E Chan
Isolation
Human periodontal pocket, Montreal, Canada
References

Makinen KK, et al. Dominance of iminopeptidase activity in the human oral bacterium Treponema denticola ATCC 35405. Curr. Microbiol. 14: 341-346, 1987.

Makinen KK, et al. Benzoylarginine peptidase and iminopeptidase profiles of Treponema denticola strains isolated from the human periodontal pocket. Curr. Microbiol. 14: 85-89, 1986.

Makinen KK, et al. Proline iminopeptidase from the outer cell envelope of the human oral spirochete Treponema denticola ATCC 35405. Infect. Immun. 64: 702-708, 1996. PubMed: 8641769

Chan EC, et al. Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and identification of new spirochete isolates from periodontal pockets. Int. J. Syst. Bacteriol. 43: 196-203, 1993. PubMed: 8494734

Cheng SL, et al. Comparative study of six random oral spirochete isolates. Serological heterogeneity of Treponema denticola. J. Periodontal Res. 20: 602-612, 1985. PubMed: 2935615

Kataoka M, et al. Characterization of amethyl-accepting chemotaxis protein gene, dmcA, for the oral spirochete Treponema denticola. Infect. Immun. 65: 4011-4016, 1997. PubMed: 9317000

Fenno JC, et al. Conservation of msp, the gene encoding the major outer membrane protein of oral Treponema spp. J. Bacteriol. 179: 1082-1089, 1997. PubMed: 9023187

Ruby JD, et al. Relationship of Treponema denticola periplasmic flagella to irregular cell morphology. J. Bacteriol. 179: 1628-1635, 1997. PubMed: 9045823

Walker SG, et al. Identification, isolation, and characterization of the 42-kilodalton major outer membrane protein (MompA) from Treponema pectinovorum ATCC 33768. J. Bacteriol. 179: 6441-6447, 1997. PubMed: 9335294

Li H, et al. Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant. J. Bacteriol. 178: 3664-3667, 1996. PubMed: 8655571

Roberts MC, et al. Characterization of tetracycline and erythromycin resistance determinants in Treponema denticola. Antimicrob. Agents Chemother. 40: 1690-1694, 1996. PubMed: 8807063

Wyss C, et al. Treponema amylovorum sp. nov., a Saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int. J. Syst. Bacteriol. 47: 842-845, 1997.

Seshadri R, et al. Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes. Proc. Natl. Acad. Sci. USA. 101(15): 5646-5651, 2004. PubMed: 15064399

Cross References

Nucleotide (GenBank) : U33210 dmcA

Nucleotide (GenBank) : L36851 Treponema denticola fliG gene, complete cds.

Nucleotide (GenBank) : L25421 Treponema denticola phosphatase gene, 5' end.

Nucleotide (GenBank) : AF082511 Treponema denticola mgl operon, partial sequence.

Nucleotide (GenBank) : U29399 Treponema denticola major outer sheath protein (msp) gene, complete cds.

Nucleotide (GenBank) : L25603 Treponema denticola (ATCC 35405) chymotrypsin-like protease gene, complete cds.

Nucleotide (GenBank) : U84257 Treponema denticola methyl-accepting chemotaxis protein B (dmcB) gene, partial cds.

Nucleotide (GenBank) : AF012922 Treponema denticola methyl-accepting chemotaxis protein (mcpA) gene, complete cds.

Nucleotide (GenBank) : AF042861 Treponema denticola OppA (oppA), putative OppB (oppB), putative OppC (oppC), putative OppD (oppD), and putative OppF (oppF) genes, complete cds.

Nucleotide (GenBank) : AF232219 Treponema denticola putative alcohol dehydrogenase, FliQ (fliQ), FliR (fliR), and FlhB (flhB) genes, complete cds; and FlhA (flhA) gene, partial cds.

Nucleotide (GenBank) : U78776 Treponema denticola gufa gene, partial cds, putative flagellar operon flgB, flgC, fliE, fliF, fliG, fliH, fliI and fliJ genes, complete cds, and fdgA gene, partial cds.

Nucleotide (GenBank) : AF122909 Treponema denticola Tap1 (tap1), flagellar hook assembly scaffolding protein FlgD (flgD), flagellar hook protein FlgE (flgE), flagellar motor protein MotA (motA), flagellar motor protein MotB (motB), FliL (fliL), flagellar switch protein FliM

Nucleotide (GenBank) : AF139203 Treponema denticola strain ATCC 35405 16S ribosomal RNA gene, partial sequence

Nucleotide (GenBank) : AE017226 Treponema denticola ATCC 35405, complete genome.