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Alicyclobacillus acidocaldarius subsp. acidocaldarius (Darland and Brock) Wisotzkey et al. (ATCC® 27009)

Strain Designations: 104-1A [CCRC 14685, CCUG 28521, CIP 106131, DSM 446, HAMBI 2071, HAMBI 2073, IFO 15652, JCM 5260, KCTC 1825, LMG 7119, NCCB 89167, NCIMB 11725, NRRL B-14509]  /  Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Bacillus acidocaldarius Darland and Brock
Strain Designations 104-1A [CCRC 14685, CCUG 28521, CIP 106131, DSM 446, HAMBI 2071, HAMBI 2073, IFO 15652, JCM 5260, KCTC 1825, LMG 7119, NCCB 89167, NCIMB 11725, NRRL B-14509]
Produces restriction endonuclease BacI
Hot spring
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Genome Sequenced Strain


Type Strain yes (type strain)
K+ uptake system
Genome sequencing strain (DOE)
Medium ATCC® Medium 573: Bacillus medium
Growth Conditions
Temperature: 55.0°C
Name of Depositor TD Brock
Special Collection National Park Service Special Collection (NPS)
Hot spring
Geographical Isolation Yellowstone National Park

Darland G, Brock TD. Bacillus acidocaldarius sp. nov., an acidophilic thermophilic spore-forming bacterium. J. Gen. Microbiol. 67: 9-15, 1971.

Wisotzkey JD, et al. Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov.. Int. J. Syst. Bacteriol. 42: 263-269, 1992. PubMed: 1374624

Michels M, Bakker EP. Low-affinity potassium uptake system in Bacillus acidocaldarius. J. Bacteriol. 169: 4335-4341, 1987. PubMed: 3624206

Bakker EP, et al. High-affinity potassium uptake system in Bacillus acidocaldarius showing immunological cross-reactivity with the Kdp system from Escherichia coli. J. Bacteriol. 169: 4342-4347, 1987. PubMed: 2957359

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

type strain

hot spring, Yellowstone National Park

hot spring

type strain

Cross References

Nucleotide (GenBank) : X62835 B.acidocaldarius amy gene for amylase.

Nucleotide (GenBank) : AJ133789 Alicyclobacillus acidocaldarius cyclomaltodextrinase gene region.

Nucleotide (GenBank) : M73834 Alicyclobacillus acidocaldarius squalene-hopene-cyclase gene, complete cds.

Nucleotide (GenBank) : AJ252160 Alicyclobacillus acidocaldarius cysA gene for putative ABC-transporter ATP-binding protein.

Nucleotide (GenBank) : AJ252161 Alicyclobacillus acidocaldarius maltose/maltodextrine transport gene region (malEFGR genes, cdaA gene and glcA gene).

Nucleotide (GenBank) : ABTB01000000 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446, whole genome shotgun sequencing project.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • This material is distributed for scientific and educational research purposes only and is part of the National Park Service Special Collection. A signed National Park Service MTA is both required prior to shipment to customers proposing a non-commercial use. A separate agreement is required from the National Park Service for customers proposing a commercial use.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation