Measure your biological processes with bioluminescent and fluorescent imaging
Reporter-labeled cell lines are useful for monitoring various biological functions and can greatly simplify your cell-based assays. These cells either constitutively or inducibly express a reporter gene (such as GFP, RFP, luciferase, or lacZ). ATCC can use a number of methods to create new cell lines that express your reporter of interest to quicken the pace of your research.
Using CRISPR/Cas9 gene editing, we have developed reporter cell lines to enable the real-time monitoring of the changing status of cells from epithelial to mesenchymal via the expression of red fluorescent protein (RFP)-tagged vimentin. These cell lines are not only an aid in dissecting the EMT/MET pathway in research field, but also are a robust platform for new cancer drug development.
To bridge the gap between in vitro and in vivo research, we can use lentiviral transduction techniques to create luciferase-labeled cell lines for biological mechanism studies and drug development. This type of reporter-labeled cell provides a relatively simple, robust, and highly sensitive means to measure biological processes and to assess drug efficacy in live animal models through bioluminescence imaging.
To simplify cell-based angiogenesis assays, we transfected GFP into hTERT-immortalized primary microvascular endothelial cells; this reporter-labeled cell line can be used to quantify angiogenic tubule formation. For many applications this cell line may provide a more reproducible and preferable alternative to methods requiring staining.
Our custom services team can help you develop reporter cell lines via multiple techniques, including but not limited to those mentioned above and many others. Contact us to discuss your research needs.
Custom Services Request
ATCC custom services
We have been the authority on cell and microbial banking for nearly 100 years. Our laboratory and storage facilities are first-in-class for the large-capacity storage of cell lines, microorganisms, and other biological materials. We can support any cell banking need and will work with you at all stages of the cell bank development process from the initiation of your project to the large-scale production of your cells and microbes.More
Cell engineering involves physical and engineering principles to control cell behavior. At ATCC, we utilize a number of cell engineering processes including hTERT immortalization and CRISPR/Cas9 gene editing for site-specific genome engineering of cell lines.More