As part of our continuing efforts to characterize and authenticate the cell lines in our collection, ATCC has developed a comprehensive database of short tandem repeat (STR) DNA profiles for all of our human cell lines.
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Short tandem repeat (STR) loci are among the most informative polymorphic markers in the human genome. STR profiles help ensure the quality and integrity of human cell lines in the scientific community. Studies have shown that a minimum of eight (8) core STR markers are required to positively identify human cell lines. Use of these 8 core STR markers (loci), D5S818, D13S317, D7S820, D16S539, vWA, TH01, TPOX, CSF1PO are recommended. These markers enable researchers to (1) show relatedness between cell lines, (2) uniquely identify human cells, and (3) for profile comparisons. Use of the 8 core loci enables a 1 x 10 discrimination rate for unrelated individuals.1
ATCC generates human STR profiles by simultaneously amplifying multiple STR loci and amelogenin (for gender determination) using the Promega PowerPlex System.2 Amplicons are separated by capillary electrophoresis and analyzed using GeneMapper Software from Thermo Fisher Scientific. Each relevant peak in the resulting electropherogram represents an allele which is alphanumerically scored and entered into the database.
The ATCC Standards Development Organization has published a consensus standard to provide the research community with a globally-accepted methodology for assessing the identity of human cell lines. The Standard also provides guidance on the interpretation of STR profiling results. This effort has facilitated the broader adoption of cell line authentication testing into cell culture laboratory operations.
After determining the STR profiles of your human cell lines, you may compare them to the human cell lines in the
Recent enhancements include:
1. Lins, A.M., et al. J. Forensic Sci. 43: 1168-1180, 1998.
2. ATCC is transitioning from the PowerPlex 1.2 System to the PowerPlex 18D System. Although the new platform interrogates 17 STR loci plus amelogenin, the additional genetic information will not be made publicly available to protect the personal identity of the tissue donor. If you have STR Profiling Tested performed by ATCC on your cell lines, your test results will provide the allele calls for all of the loci evaluated.
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