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Interrogating the STR Database

Digital display of DNA markers.

Learn how to query the database and interpret the results

How to interrogate the STR database

  • Log in to the ATCC website to access the STR database query form
  • For each query, enter either (1) an ATCC catalog number, OR (2) at least 7 of the 8 STR loci plus amelogenin (AMEL) of the cell line you would like to compare against the ATCC STR Profile database. The more information that you can provided, the more accurate the results generated by the matching algorithm.
    • Separate each allele entry with a comma (eg, CSF1PO = 11, 12); a space after the comma is not required. Single allele entries are accepted (eg, Amelogenin (AMEL) = X, TPOX =8)
    • Blank entries at any loci will not be counted
    • The amelogenin gene is a genetic marker used for gender determination and is not comprised of STR units.
    • Log in to the ATCC website to access the STR database query form.
    • Click either the 'Limited Search' or 'Expanded Search' button.
      1. Limited Search: The search will return matches that are ≥ 80%. This level of matching indicates that the cell lines are related.
      2. Expanded Search: The search will return matches that are ≥ 56%. The level of matching from 79% to 56% suggests that additional analysis must be employed to confirm the relatedness of the test cell line.

How to interpret results

  • The “ATCC Number” field is given precedence if data is entered into both the “ATCC Number” field and the STR loci field.
  • Results are presented in descending order based on the highest percent match to the query.
    1. Your cell line is considered to be “identical” to a culture in the ATCC STR database when the entered STR profile yields a 100% match (all 8 of the 8 loci) to the result set.
    2. Your cell line is considered “related“ to a culture in the ATCC STR database when the STR profile entered yields matches that are ≥ 80% but less than 100%
    3. Your cell ilne that results in a match from 79% to 56% suggests that additional analysis, by other methods, must be employed to confirm the relatedness of the 'test' cell line to other cultures held by ATCC.

As in the past, when we find a misidentified cell line among our holdings (i.e., the DNA profile is similar or identical to that of an unrelated cell line), we will post a note on the Reclassified Cell Lines page of our website.

 

Disclaimer:
Reference to this database and the data contained therein may be cited in publications, and ATCC encourages such citation or reference. While every reasonable effort has been made to assure the accuracy of these data, no warranty, express or implied, is made by ATCC as to their accuracy.

While ATCC has largely used the Promega PowerPlex® 1.2 System in the creation of these data and recommends that researchers wishing to produce data for comparison also use a Promega PowerPlex®System ATCC does not provide a general endorsement of this product or provide any warranty or representation regarding its quality or performance in the scientific community for the identification of human cell lines.

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