American Type Culture Collection (ATCC) Logo American Type Culture Collection (ATCC) Logo 0
  • Quick Order
  • Careers
  • Support

Advancements in Human Cell Line Cryopreservation for Assay-Ready Efficiency

October 10, 2024, at 12:00 PM ET

Abstract

Cell-based bioassays are used across numerous biotechnology industries for various applications such as drug screening, vaccine development, and quality control. However, developing and deploying bioassays can be challenging due to the lengthy processes of cell culture. For instance, generating working cell banks and post-thaw recovering cryopreserved vials each require careful consideration and allocation of resources. ATCC scientists created a streamlined solution that eliminates the challenges posed by banking cells for cell-based assays. Our assay-ready cells can be used directly in cell-based assay from frozen, without the need for additional cell culture or expansion.

Here, we present application data for the ATCC Assay Ready Cells THP-1-AR (ATCC TIB-202-AR) and THP-1-NFκB-Luc2-AR (ATCC TIB-202-NFkB-LUC2-AR). Upon thaw, the cells feature high viability, fast recovery, and the ability to differentiate into macrophage-like cells that express the appropriate markers and functional attributes. These data demonstrate an assay-ready format that reduces culture time without diminishing biological relevance and has the potential to standardize and replace working cell banks for more repeatable results.

Key Points

  • Generating working cell banks and post-thaw recovering cryopreserved vials presents a great challenge in the drug development process
  • ATCC scientists generated a solution that allows you to go from frozen to data in one work day
  • ATCC Assay Ready Cells can be used directly in cell-based assay from frozen, without the need for additional cell culture or expansion
  • The thawed cells show high viability, fast recovery, and exhibit the ability to differentiate into macrophage-like cells that express the appropriate markers and functional attributes

Presenters

Lukas Underwood.jpg

Lukas Underwood, PhD

Scientist, BioNexus Cryobiology, ATCC

Dr. Underwood specializes in mammalian cell preservation and characterization. He has a PhD in Mechanical Engineering and Applied Sciences from the University of Michigan with a focus in biological engineering. Lukas has extensive experience in and spectrographic and thermodynamic characterization of mammalian cell preservation and formulation development.

Dr. Underwood joined ATCC is March 2022 and is working on the development of novel preservation formats for Cell Biology products. Since joining ATCC, Dr. Underwood has collaborated with and consulted for several of the Cell Biology R&D groups located on the Gaithersburg campus.

Diana Douglas, headshot.

Diana Douglas, BS

Lead Biologist, ATCC

Diana Douglas is a Lead Biologist at ATCC. For the last nine years, she has focused her research on the development of advanced biological models including the use of CRISPR/Cas9 gene-editing technology. Previously, Ms. Douglas worked at the Baker Institute for Animal Health at Cornell University and the Dalton Cardiovascular Research Center at the University of Missouri, where her research focused on the mechanisms of necrotic cell death in heart disease. Ms. Douglas attended Truman State University where she obtained a Bachelor of Science in Biology.

Register