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Simplifying Assay Development with Molecular Standards: Remove Culturing from the Equation

Jun 17, 2021 at 12:00 PM ET

Abstract

Reliable controls are a critical component in the development of molecular-based assays. However, the extraction, preparation, and validation of nucleic acids can be time and labor intensive. Further, many microorganisms can be difficult to culture or may require a high-containment facility, which requires additional expertise and equipment. To address this need, ATCC has developed a portfolio of genomic and synthetic molecular standards that provide convenient, easy-to-use, reliable controls for quantitative PCR applications. These highly validated process controls remove the burden of culture work, nucleic acid extraction, and quantitation. In this webinar, we will cover ATCC’s growing offering of molecular standards—from human pathogens to microbiome components—and we will provide application data demonstrating the potential use of these products in diagnostic assay development.

Key Points

  • Assay accuracy and precision depends heavily on using faithful standards in the development process. 
  • The use of molecular standards reduces the required cost and labor involved with culture, extraction, and quantitation processes.
  • ATCC validated molecular standards cover a broad range of organisms, from human pathogens to microbiome components.

Watch The Presentation

Presenter

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Kyle Young, MBA

Product Line Business Specialist, ATCC

Kyle Young, MBA, is a Lead Biologist with 14 years of laboratory experience in virology and molecular biology. He has worked extensively with virus authentication at ATCC, leading several process development and improvement efforts. He has also been involved in the attainment of ISO certifications in several laboratories. He currently performs product line management work for ATCC’s Microbiology collections. Mr. Young earned a BS in Biology from the University of Tennessee and an MBA from George Mason University.

Molecular Assay Development Resources

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Challenges and solutions in assay development

While molecular-based assays have exhibited numerous benefits over culture-based approaches, they can be challenging to develop and validate. Read our white paper to explore the common problems faced in assay development and how standards can help.

Download the Whitepaper
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Molecular Analysis

If you can't find the nucleic acids you need, we can grow, extract, and purify nucleic acids from most ATCC cultures in larger quantities. Rely on ATCC for molecular analysis, viral quantitation, and next-generation sequencing.

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