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Prostate Cancer credit NCI

Restoring Reproducibility in Research: Four Tools to Authenticate Your Cells

Nov 11, 2021 at 12:00 PM ET

Abstract

The lack of reproducibility in life science research is a far-reaching crisis; cell line misidentification and contamination profoundly impacts biomedical studies and can result in publication retraction, credibility loss, data invalidation, and financial cost. In this webinar, you will learn how you can help restore reproducibility in research by using ATCC’s portfolio of authentication services and products, including human and mouse STR profiling, mycoplasma detection, and cytochrome 1 oxidase barcoding for species identification. After the presentation, join us for a lively discussion as ATCC cell line authentication experts answer your questions.

Key Takeaways

  • Irreproducible research can result in publication retraction, credibility loss, data invalidation, and financial cost.
  • ATCC provides several services and products, such as human and mouse STR profiling, mycoplasma detection, and cytochrome 1 oxidase barcoding, for cell line authentication.
  • Including a rigorous program of cell authentication as part of your research plan can help ensure the validity of your experimental results.

Watch The Presentation

Presenter

Kevin Grady, headshot.

Kevin Grady, BS

Senior Product Line Business Manager, ATCC

Kevin Grady is the Senior Product Line Business Manager for Cell Biology at ATCC. He has been with ATCC for 8 years; prior to ATCC, he held positions at Lonza as Global Product Manager and Director of Scientific Support. Kevin has a long history in the life science industry additionally serving as Director of Scientific Support at Amaxa and Manager of Technical Support at Life Technologies. Mr. Grady has always found great satisfaction in helping researchers learn how to use available products and tools to be more productive and successful in reaching their research goals.

Cell authentication tests

Green K562-GFP with nuclear stain.

Human Cell STR Testing

ATCC authenticates human cells through its easy-to-use human cell short tandem repeat (STR) profiling service. Once you receive your FTA Sample Collection Kit, spot your cells onto Whatman FTA cards, let them dry, and mail them to ATCC.

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Purple and green top mouse skin cancer cells.

Mouse Cell STR Testing

Developed by ATCC in partnership with the National Institutes for Standards and Technology (NIST) and tested by 10 independent laboratories, ATCC’s Mouse STR profiling service offers a new global standard for mouse cell line authentication.

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Scientist looking into a microscope, hand on one of the eye pieces.

Mycoplasma Testing

ATCC offers two options for testing for mycoplasma, the notoriously common bacteria that quickly infiltrate and destroy cell cultures: PCR-based mycoplasma testing or culture-based testing (Hoechst DNA staining and direct culture testing).

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