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pR16.11 purified plasmid DNA

VRMC-8

Organism
HRV type 16 molecular clone
Clone type
Clone
Applications
Molecular biology
Product format
Dried
Shipping information
Plasmid DNA
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
Contains full-length sequence

Characteristics

Comments

This full-length infectious clone is not published yet although the sequence was published (Virus Gene 9:177-181, 1995).


The first base of the HRV 16 genome was ligated to the last base of the T7 promoter. The HRV 16 genome has a poly (A) track of 52 A's at the 3' end. 


The full-length T7 transcripts of HRV 16 generated from this construct had a specific infectivity of about 5 x 106 PFU/µg, which is similar to the virion RNA.


The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A. RefLee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280

Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
9.460
Vector name
pUC19 + T7 promoter
Type of vector
E. coli plasmid
Vector information
Host required: E. coli MV1193
Host medium: LB broth with 50 µg/mL ampicillin (ATCC Medium #1227)
Vector size (kb): nucleotides 1 to 400 and 2678 to 2686 were deleted in the cloning process.
Cloning sites
BamHI
Markers
ampR

Insert information

Insert size (kb)
7.1200000000000001
Insert information
Insert: Human rhinovirus (HRV) type 16 cDNA
ClassificationPicornavirus, Rhinovirus, Human
Insert description (full-length, etc.):  full-length HRV 16 cDNA
Full/partial sequence available (ie. GenBank #): L24917, with differences noted (J. Virology 77: 6235-6244, 2003)
Markers (genes and/or restriction sites):
Enzyme  # sites  Fragment Length  Location of Cuts
BamH1        2          6455; 3005         
Pst1             3     6298; 2365; 527
Kpn1           3     4452; 4002; 1006
BgIII           4    5085;2045;1864;466

Handling information

Handling procedure
  1. Rehydrate plasmid with water or TE buffer.
  2. Plasmid can be transformed into a suitable E. coli host using standard protocols and then grown in LB + 50 µg/mL amplicillin.
  3. Incubate cultures at 37°C.
  4. Isolate DNA using standard plasmid preparation procedures.
Handling notes

The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A.

- J. Virology 77: 6235-6244, 2003

History

Deposited as
Human rhinovirus type 16
Depositors
W Lee
Special collection
NCRR Contract
Cross references
GenBank L24917

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441

Lee WM, et al. Complete sequence of the RNA genome of human rhinovirus 16, a clinically useful common cold virus belonging to the ICAM-1 receptor group. Virus Genes 9: 177-181, 1995. PubMed: 7732663

Lee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280

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