pR16.11 purified plasmid DNA

This full-length infectious clone is not published yet although the sequence was published (Virus Gene 9:177-181, 1995).

The first base of the HRV 16 genome was ligated to the last base of the T7 promoter. The HRV 16 genome has a poly (A) track of 52 A's at the 3' end. 

The full-length T7 transcripts of HRV 16 generated from this construct had a specific infectivity of about 5 x 10⁶ PFU/µg, which is similar to the virion RNA.

The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A. RefLee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280

1. Rehydrate plasmid with water or TE buffer.
2. Plasmid can be transformed into a suitable E. coli host using standard protocols and then grown in LB + 50 µg/mL amplicillin.
3. Incubate cultures at 37°C.
4. Isolate DNA using standard plasmid preparation procedures.

The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A.

- J. Virology 77: 6235-6244, 2003

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