pR16.11 purified plasmid DNA
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This full-length infectious clone is not published yet although the sequence was published (Virus Gene 9:177-181, 1995).
The first base of the HRV 16 genome was ligated to the last base of the T7 promoter. The HRV 16 genome has a poly (A) track of 52 A's at the 3' end.
The full-length T7 transcripts of HRV 16 generated from this construct had a specific infectivity of about 5 x 106 PFU/µg, which is similar to the virion RNA.
The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A. RefLee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280
The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A.
- J. Virology 77: 6235-6244, 2003
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Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955
Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441
Lee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280