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Human mastadenovirus F


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Human mastadenovirus F strain Dugan [79-18025] is propagated in 293 [HEK-293] cells (ATCC CRL-1573). This strain was isolated in 1979 from the feces of a human with infantile gastroenteritis. This product is whole-genome sequenced has applications in enteric and infectious disease research.
Product category
Product type
Animal virus
Adenoviridae, Mastadenovirus, Human mastadenovirus F
Strain designation
Dugan [79-18025]
Genome sequenced strain
Isolation source
Feces, infantile gastroenteritis.
Geographical isolation
Enteric disease research
Infectious disease research
Product format
Storage conditions
-70°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Enteric Research


This virus is a good immunogen in rabbit and horse. There are no cross-reactions with 37 prototypes by serum neutralization (SN) and hemagglutination inhibition (HAI). This strain is related to the Tak strain as indicated by SN and HAI. At low titer, the virus hemagglutinates rat red blood cells (RBC). It does not hemagglutinate rhesus monkey or human type O RBC. The virus is stable at -20°C.
Mycoplasma contamination
Not detected

Handling information

293 [HEK-293] (ATCC CRL-1573)
cell clustering; cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
95% Air, 5% CO2
3-7 days, a 5% CO2 in air atmosphere is recommended
Recommendation for infection
For best results, cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent)
Handling notes
Good immunogen in rabbit and horse; no cross-reactions with 37 prototypes by SN and HAI. Related to strain Tak by SN and HAI. HA at low titer with rat RBC; no HA with rhesus monkey or human O RBC. Stable at -20°C.
Key abbreviations
293, Human embryonic kidney cells
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum

Quality control specifications

1.0 mL
Verification method
Whole-genome Sequencing


Deposited as
Adenovirus type 40
JC de Jong
Type of isolate
Year of origin
Cross references
GenBank NC_001454 Human adenovirus type 40.
GenBank L19443 Human adenovirus type 40, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

de Jong JC, et al. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. J. Med. Virol. 11: 215-231, 1983. PubMed: 6306161

de Jong JC. Abstr. V Int. Cong. Virol. : 194, 1981.

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