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Human mastadenovirus B


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Product category
Product type
Animal virus
Adenoviridae, Mastadenovirus, Human mastadenovirus B
Strain designation
Common name
Human adenovirus B
Genome sequenced strain
Isolation source
Lung and kidney tissues from 61-year-old woman who died with diffuse interstitial pneumonia.
Infectious disease research
Respiratory disease research
Product format
Storage conditions
-70°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Respiratory research


Acid and chloroform stable, partially heat-labile, no cationic stabilization. Group CF (hexon) titers of 1:16-1:128 expected.
Mycoplasma contamination
Not detected

Handling information

cell clustering; cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)
95% Air, 5% CO2
2 to 4 days, a 5% CO2 in air atmosphere is recommended.
Recommendation for infection
For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Handling notes
HA with rhesus and vervet RBC (typically 1:1024-1:8192). No HA with other RBC. Virion 75nm in diameter, excluding fibers. Low SN cross between AV-11 virus and AV-35 sera. Moderate HAI cross between AV-35 virus and AV-34 sera. High HAI cross between AV-34 virus and AV-35 sera.

Key abbreviations
°C, Degrees Celsius
AV, Adenovirus
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
HA, Hemagglutination
HAI, Hemagglutination inhibition
HAm, Human amniotic cells
HEp-2, Human laryngeal tumor cells
RBC, Red blood cell
SN, Serum neutralization

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Adenovirus type 35
JC Hierholzer
Type of isolate
Patient age
61 years
Patient gender
Cross references
GenBank U52572 Human adenovirus type 35 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Myerowitz RL, et al. Fatal disseminated adenovirus infection in a renal transplant recipient. Am. J. Med. 59: 591-598, 1975. PubMed: 170822

Stalder H, et al. New human adenovirus (candidate adenovirus type 35) causing fatal disseminated infection in a renal transplant recipient. J. Clin. Microbiol. 6: 257-265, 1977. PubMed: 71305

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