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Genomic DNA from Human herpesvirus 5 strain AD-169


Viral nucleic acids, including virus genomic DNA, in total DNA isolated from MRC-5 cells (ATCC® CCL-171™™) infected with Human herpesvirus 5 (HCMV) strain AD-169 (ATCC® VR-538™™).Virus and host cells are also available through the ATCC® Catalog.  This product was prepared using methods known to inactivate viruses.
Product category
Product type
Nucleic acid
Derived from
Human herpesvirus 5 AD-169 (ATCC VR-538)
Human herpesvirus 5, Cytomegalovirus
Genome sequenced strain
Blood-borne disease research
Infectious disease research
1 µg/mL
100 μL
Product format
Shipping information
Total DNA from infected cells
Storage conditions
-70°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.


This product is a preparation of viral nucleic acids, including virus genomic DNA, in total DNA from MRC-5 cells (ATCC® CCL-171™.) infected with Human herpesvirus 5 (HCMV) strain AD-169 (ATCC® VR-538™). The product is supplied at 100 ul per vial, shipped frozen. It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Strain AD-169 has been completely sequenced. Genbank: X17403

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation of the DNA.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
DNA isolated from virally infected cells is appropriate for PCR and other molecular virology applications.

Quality control specifications

Total amount
Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.
Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µL of product on a 0.8% agarose gel, visualized by ethidium bromide staining.
Functional tests
Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.
Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon.
Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.


Cross references
GenBank X17403 Human cytomegalovirus strain AD169 complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Viral DNA from infected cells

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