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Human herpesvirus 5

VR-538

Product category
Viruses
Product type
Animal virus
Classification
Herpesviridae, Cytomegalovirus
Strain designation
AD-169
Common name
Human cytomegalovirus
Type strain
No
Genome sequenced strain
Yes
Isolation source
Adenoid tissue.
Applications
Infectious disease research
Sexually transmitted disease research
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Testing virucides
Sexually Transmitted Disease Research

Characteristics

Comments
Preserved in virus growth medium with 7% DMSO.
GenBank: X17403.
Mycoplasma contamination
Not detected

Handling information

Host
Effects
cell enlargement; cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC® 30-2003™) + 10% FBS (ATCC® 30-2020™ or 30-2021™)
Temperature
36°C
Atmosphere
95% Air, 5% CO2
Incubation
4 to 11 days
Recommendation for infection
This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.5 - 2.0 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1 to 1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Handling notes
Stable for 6 months at -60°C in the form of glycerinated cells.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
DMSO, Dimethyl sulfoxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection

Quality control specifications

Volume
1.0 mL

History

Deposited as
Cytomegalovirus
Depositors
WA Chappell
Type of isolate
Human
Patient age
7 years
Patient gender
Female
Special collection
NCRR Contract
Cross references
GenBank X17403 Human cytomegalovirus strain AD169 complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Huang L, et al. The variable e' endds of a human cytomegalovirus oriLyt transcript (SRT) overlap an essential, conserved replicator element. J. Virol. 70: 5272-5281, 1996. PubMed: 8764037

Iskenderian AC, et al. Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes. J. Virol. 70: 383-392, 1996. PubMed: 8523552

Noraz N, et al. Human cytomegalovirus-associated immunosuppression is mediated through interferon-alpha. Blood 89: 2443-2452, 1997. PubMed: 9116288

View All Curated Citations for this Product

Frequently Asked Questions

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Telephone

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