Quantitative Genomic RNA from Severe acute respiratory syndrome-related coronavirus 2 strain USA/MD-HP05285/2021 (Delta variant)

Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/MD-HP05285/2021.

This isolate is lineage AY.24 (Pango v.3.1.14 2021-09-28), Delta (B.1.617.2-like) (Scorpio), and GISIAD clade GK using Phylogenetic Assignment of Named Global Outbreak lineages (PANGO) tool.  Due to the dynamic nature of lineage determination using PANGO, discrepancies may exist between vial labels and the current lineage assignment.

The complete genome of the clinical isolate of SARS-CoV-2, hCoV-19/USA/MD-HP05285/2021 has been sequenced (GISAID: EPI_ISL_2103264). The following mutations are present in the clinical isolate: Spike D614G, Spike D950N, Spike L452R, Spike P681R, Spike T19R, Spike T478K, M (Membrane protein) I82T, N (Nucleocapsid) D63G, N D377Y, NS3 S26L, NSP3 (Non-structural protein 3) P822L, NSP4 (Non-structural protein 4) A446V, NSP6 (Non-structural protein 6) T181I, NSP6 V149A, NSP10 (Non-structural protein 10) L133F, NSP12 (Non-structural protein 12) G671S, NSP12 P323L, NSP13 (Non-structural protein 13) P77L. It was labelled as VOC Delta GK/478K.V1 (B.1.617.2+AY.x), first detected in India by the World Health Organization (WHO).

Genomic RNA from SARS-CoV-2 must be handled at BSL-2 containment.

Acknowledgment for publications should read "The following reagent was deposited by the Centers for Disease Control and Prevention and obtained through BEI Resources, NIAID, NIH: Genomic RNA from SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP05285/2021 (Lineage B.1.617.2; Delta Variant), NR-56127."

1. Thaw the vial on ice. Avoid exposing the RNA to repeated freeze-thaw cycles as it may result in degradation.
2. Gently mix the sample to ensure an even distribution of material.
3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.

RNA is easily degraded. Take extra precautions against contamination by using new gloves and clean lab coats when working with RNA. Use only RNase-free lab materials when handling this product. Vortexing can damage the RNA. Gentle pipetting is highly recommended. Aliquoting is highly recommended to avoid multiple freeze-thaws, which can damage the RNA.

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