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Human adenovirus 15

VR-16

Product category
Viruses
Product type
Animal virus
Classification
Adenoviridae, Mastadenovirus, Human adenovirus D
Strain designation
305 [955, CH. 38]
Isolation source
Conjunctival scrapings from a patient with trachoma
Geographical isolation
Saudi Arabia
Applications
Infectious disease research
Respiratory disease research
Product format
Freeze-dried
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Respiratory research

Characteristics

Mycoplasma contamination
Not detected

Handling information

Host
Host Range:  HEK, HeLa, KB and other human cell lines; human embryonic kidney cells
Effects
CPE
Incubation
2-3 days
Handling notes
May require passage with large inocula before satisfactory titer is obtained.  Agglutinates both rat and rhesus RBC but usually the former to a higher titer than the latter.  Similar or identical to adenovirus type 25 in neutralization but distinguishable in HAI tests.  Contains soluble group-specific CF antigen

Reconstitute to original volume with distilled water and store at -60°C or colder.
Key abbreviations
EMEM, Eagle's Minimum Essential Medium
RBC, Red blood cell
TC, Tissue culture
TCID[50], Median tissue culture infective dose
CF, Complement fixation
CPE, Cytopathic effect
HAI, Hemagglutination inhibition
HEK, Human embryonic kidney cells
HeLa, Human cervical carcinoma cells
KB, Human oral epidermal carcinoma cells
LN, Human lymph node cells

History

Deposited as
Adenovirus type 15
Depositors
M Schaeffer, DA Stevens
Type of isolate
Human
Year of origin
1955
Cross references
GenBank AF086567 Human adenovirus type 15 early E3B 10.4 kDa protein, early E3B 14.5 kDa protein, and early E3B 14.7 kDa protein genes, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Murray ES, et al. Adenoviruses from Canadian cases of keratocon- junctivitis. Am. J. Ophthalmol. 43: 32-35, 1957.

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Frequently Asked Questions

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