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Adeno-associated virus 5


Adeno-associated virus 5 is propagated in LLC-MK2 Derivative cells (ATCC CCL-7.1) with a helper adenovirus. This strain was isolated from a human clinical specimen.
Product category
Product type
Animal virus
Parvoviridae, Dependovirus, Adeno-associated virus 5
Strain designation
Type strain
Isolation source
Human clinical specimen
Product format
Storage conditions
-70°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Suggest ATCC VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells.


Depends upon adenovirus for replication. Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells. *Helper virus may be inactivated by heating to 56C for 30 min.
Mycoplasma contamination
Not detected

Handling information

LLC-MK2 Derivative (ATCC CCL-7.1)
cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020) + ATCC VR-846 Adenovirus 2
95% Air, 5% CO2
3 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.
Recommendation for infection
For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Handling notes
Depends upon adenovirus for replication. Suggest ATCC VR-846, Adenovirus 2, as helper virus, which produces CPE on ATCC CCL-7.1 cells. Helper virus may be inactivated by heating at 56°C for 30 minutes.
Key abbreviations
AAV, Adeno-associated virus
bp, Base pairs
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
HeLa, Human cervical carcinoma cells
KB, Human oral epidermal carcinoma cells
LLC-MK2, Rhesus monkey kidney cells
mL, Milliliter
PCR, Polymerase chain reaction
TC, Tissue culture
TCID[50], Median tissue culture infective dose
VP1, capsid protein 1


Deposited as
Adeno-associated virus 5
U Bantel-Schaal, H zur Hausen, U Bantel-Schaal
Type of isolate
Special collection
NCRR Contract
Cross references
GenBank AF085716 Adeno-associated virus 5 DNA binding trs helicase (Rep22) and capsid protein (VP1) genes, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

or colder

Georg-Fries B, et al. Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. Virology 134: 64-71, 1984. PubMed: 6200995

Bantel-Schaal U, zur Hausen H. Characterization of the DNA of a defective human parvovirus isolated from a genital site. Virology 134: 52-63, 1984. PubMed: 6324476

can also be grown on other human and rodent cell lines

penile flat condylomatous lesion

View All Curated Citations for this Product

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