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Influenza A virus (H1N1)

VR-1469

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Influenza A virus (H1N1) strain A/PR/8/34 is propagated in MDCK (NBL-2) cells (ATCC CCL-34). This strain was isolated in 1934 from a human patient in Puerto Rico and was deposited by the Centers for Disease Control and Prevention. Use this strain in your respiratory disease research.
Product category
Viruses
Product type
Animal virus
Classification
Orthomyxoviridae, Influenzavirus A
Strain designation
A/PR/8/34
Type strain
No
Genome sequenced strain
Yes
Isolation source
Patient, Puerto Rico.
Geographical isolation
United States
Applications
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Influenza Research

Characteristics

Comments
ATCC VR-1469 is a TC-adapted item derived from virus passaged in eggs (ATCC VR-95).
Mycoplasma contamination
Not detected

Handling information

Host
MDCK (NBL-2) (ATCC CCL-34)
Effects
CPE; cell degeneration; cell rounding; cell sloughing
Complete medium
EMEM (ATCC 30-2003) + 1 mM HEPES (Gibco® 15630-080) + 0.125% BSA Fraction V (Gibco® 15260-037) + 1 µg/mL TPCK-treated Trypsin (USB® 22725)
Temperature
35°C
Atmosphere
95% Air, 5% CO2
Incubation
2-4 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes
ATCC VR-1469 is a TC-adapted item derived from virus passaged in eggs (ATCC VR-95).
Key abbreviations
BSA, Bovine serum albumin
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
MDCK, Madin Darby canine kidney cells
MOI, Multiplicity of infection
PBS, Phosphate-buffered saline
TPCK, L-1-tosylamido-2-phenylethyl chloromethyl ketone

Quality control specifications

Volume
1.0 mL
Verification method
Whole-genome Sequencing

History

Deposited as
Influenza A virus
Depositors
ATCC, M Coleman, Centers for Disease Control and Prevention
Type of isolate
Human
Year of origin
1934
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935.

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico

800-638-6597

Outside the US

+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time