TSD-283 ™
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Grow in supplemented basal medium at 25-45°C for 2-3 days at pH of 5.2-6.0 when inoculated in exponential phase.
Supplemented Basal Medium
Per liter:
NaCl 1.1 g
NH4Cl 474 mg
CaCl2 2H20 100 mg
MgCl2 6H20 65 mg
KH2PO4 150 mg
Na2CO3 32 mg
Trace minerals Wolfe’s soln. 20 mL
Na-butyrate 3.3 g
DI H2O 856 mL
Sparge 30-40min N2:CO2 (80:20)
L-cysteine HCl 470 mg
FeCl2 (10g/L stock) 0.125 mL
Autoclave 121 °C, 20 min
Yeast extract (10% w/v)a 20 mL
Fructose or glucose (250 g/L)a 22 mL
Vitamin solution 2xa 2 mL
MES 1Ma 100 mL
Adjust to pH 5.5-6.5
a filtered sterilized
2x vitamin solution
Per liter:
pyridoxine hydrochloride 20 mg
riboflavin 10 mg
nicotinic acid 10 mg
folic acid 4 mg
calcium pantothenate 10 mg
p-amino benzoic acid 10 mg
d-biotin 4 mg
thiotic acid 10 mg
vitamin B12 10 mg
thiamine HCl 10 mg
2-mercaptoethanesulfonic acid 4 mg
Trace minerals Wolfe' modified
Per liter:
EDTA 0.5 g
MgCl2 x 6H2O 3.63 g
MnCl2 x 4H2O 427.1
NaCl 1m gg
FeCl2 219.3
CoCl2 x 6H2O 180 mg
CaCl2 (anhydrous) 0.1 g
ZnCl2 210.9
CuCl2 x 2H2O 14.64
H3BO3 0.010 g
Na2MoO4 x 2H2O 0.010 g
Na2SeO3 (anhydrous) 0.001 g
Na2WO4 x 2H2O 0.010 g
NiCl2x 6H2O 0.020 g
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ATCC may fully accessions new type strains into its general culture collection. At that time, ATCC will provide an “ATCC” designation to the strain, fully characterize the strain, and provide a Certificate of Analysis with authentication data for that specific item.
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Esquivel-Elizondo S, Bağcı C, Temovska M, et al. The Isolate Caproiciproducens sp. 7D4C2 Produces n-Caproate at Mildly Acidic Conditions From Hexoses: Genome and rBOX Comparison With Related Strains and Chain-Elongating Bacteria. Front Microbiol 11: 594524, 2021. PubMed: 33584563
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