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Mouse ES Cell Basal Medium

SCRR-2011

ATCC® Mouse ES Cell Basal Medium is a sterile liquid tissue culture medium containing phenol red that has been optimized to support the undifferentiated self-renewal cycle related to mouse embryonic stem cell growth.

The medium contains a stable glutamine dipeptide, providing a long shelf-life when handled and stored properly. The medium may be supplemented by addition of ES-Cell Qualified Fetal Bovine Serum (FBS) or a serum replacement designed for mouse ES cells.

This medium can support the growth of mouse ES cells on either a mitotically inactivated layer of mouse embryonic fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.
Applications
Stem cell research
Cell growth and viability
Volume
500 mL
Storage conditions
2°C to 8°C
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Documentation

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Detailed product information

General

Specific applications
General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

Handling information

Handling procedure
Preparation of Complete Growth Medium for Mouse ES Cells 
Grow ES cells in Mouse ES Cell Basal Medium that has been supplemented with the following components:
  • 0.1 mM 2-mercaptoethanol (Invitrogen Cat. No. 21985) 
  • 1,000 U/mL mouse leukemia inhibitory factor (LIF) (Chemicon Cat. No. ESG1107) 
  • 10% to 15% ES-Cell Qualified FBS (ATCC SCRR-30-2020) or an ES cell qualified serum replacement 

Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Osmolality
260 to 285 mOsm/kg
pH
7.1 to 7.5

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

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