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pCarrier 8 (integ)

SB-1005

pCarrier 8 (integ) is an integration vector for targeting the yeast Saccharomyces cerevisiae HO locus. Two 45 bp unique nucleotide sequences (UNS-1: GGTTTACCGAGCTCTTATTGGTTTTCAAACTTCATTGACTGTGCC and UNS-X: GGTTAGGCGACTGTTATAACTTACCTCTGTAATACTAGTGATACC) in the vector allow for the assembly of multiple transcription units. Two HO fragments (~480 bp) flanking these sequences mediate HO locus integration via homologous recombination (detail information is described in the ATCC® Synthetic Biology Solutions User Guide).

Product type
Yeast
Applications
Genetic engineering
Industrial biotechnology
Storage conditions
2°C to 8°C
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Price: $550.00 ea
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Molecular Grade Water

60-2450

Price: $40.00 ea

Detailed product information

General

Specific applications
A carrier vector in the ATCC® Synthetic Biology Yeast Tool Kit designed for use in the construction of a single transcription unit and gene circuit.

Characteristics

Comments
pCarrier 8 (integ) is an integration vector for targeting the yeast Saccharomyces cerevisiae HO locus

Vector information

Construct size (kb)
2.926
Type of vector
Carrier vector
Markers
camR

Handling information

Handling procedure

Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).

Quality control specifications

Volume
2 μg to 3 μg

History

Depositors
R Weiss, Massachusetts Institute of Technology

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Ron Weiss, personal communication

Frequently Asked Questions

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Telephone

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